Fibronectin rat plasma

EC Number:	289-149-2
MDL number:	MFCD00131062
eCl@ss:	42020141
NACRES:	NA.75

Кат. номер

F0635-.5MG

F0635-1MG

F0635-2MG

F0635-PH

F0635-1MG-PW

F0635-2MG-PW

F0635-.5MG-PW

Fibronectin (FN)/FN1 (fibronectin 1) is a multifunctional glycoprotein, produced by hepatic stellate cells (HSCs). It is a constituent of extracellular matrix (ECM). FN1 is found in the cell membrane and cytoplasm. It is also synthesized and liberated by fibroblasts, endothelial cells, macrophages and hepatocytes in vivo.
Fibronectin rat plasma has been used in the induction of differentiation , adhesion to extracellular matrix, neural differentiation and primary neuronal ventral mesencephalon cultures.
Biochem/physiol Actions
Fibronectin (FN)/FN1 (fibronectin 1) plays a major role in cell cycle progression, cell adhesion and proliferation and fibrotic progression. It is essential for for collagen matrix assembly. High expression of FN has a vital role during liver fibrosis. FN1 helps in the support of other matrix proteins.
Components
Fibronectin is an adhesive glycoproteins found in both the cell surface and in plasma.
Caution
The reconstituted solution should be stored in working aliquots at -20°C or lower. Vortexing, excessive agitation, or additoinal freezing and thawing of reconstituted fibronectin are not recommended.
Preparation Note
This product is lyophilized from 0.05 M Tris buffered saline at pH 7.5. It is soluble at 1 mg/mL in water, and should be allowed to dissolve for at least 30 minutes at 37°C. A small amount of undissolved material may remain, but it will not affect performance. The protein homogeneity is evaluated by immunoelectrophoresis, showing a single arc, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B and hepatitis C.
In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C.
Analysis Note
A single arc is shown when reacted with anti-fibronectin by IEP (immunoelectrophoresis).

Quality Level	200
biological source	rat plasma
sterility	sterile
product line	BioReagent
form	powder
packaging	pkg of 0.5 mg","pkg of 1 mg","pkg of 2 mg
application(s)	cell culture | mammalian: suitable
surface coverage	15 µg/cm2
solubility	H2O: 1 mg/mL at 37 °C, clear, colorless (some particles)
UniProt accession no.	P04937,
shipped in	dry ice
storage temp.	20°C
Gene Information	rat ... Fn1(25661)

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable