Tris-Borate-EDTA buffer

MDL number:	MFCD00236358
PubChem Substance ID:	329826793
NACRES:	NA.25

Кат. номер

T4415-100ML-PW

T4415-10L-PW

T4415-1L-PW

T3038-1L-PW

T4415-10L

T4415-20L

T4415-PH

T4415-1L

T4415-4L

T4415-100ML

T4415-200L

TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated, water-soluble, and protected from degradation. This concentrate can be easily diluted to 1x or 0.5x before use (with molecular biology grade water).
Ready for use in gel electrophoresis after dilution to working concentrations.
Tris-Borate-EDTA buffer has been used for pulsed-field gel electrophoresis (PFGE).
TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids.
Dilution of the TBE stock concentrates to a 1x TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5x or 10x stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
The 4L, 10L and 20L sizes are supplied in dispenser with a spigot.
Prepared with 18 megohm water
Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).
TBE buffer is prone to precipitation over time. Precipitation generally will not adversely affect performance.

Quality Level	200,
product line	BioReagent
impurities	DNase and RNase, none detected
suitability	suitable for electrophoresis
SMILES string	OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI	1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H
InChI key	OSBLTNPMIGYQGY-UHFFFAOYSA-N

pictograms	GHS08
signalword	Danger
hcodes	H360FD
pcodes	P201 - P202 - P280 - P308 + P313 - P405 - P501
Personal Protective Equipment	Eyeshields,, Gloves,, type ABEK (EN14387) respirator filter,
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable