Cell Lines & Specialty Cell Culture
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MRC-7
Кат. номер 85020203-DNA-5UG 85020203-RNA-5UG 85020203-1VL 85020203-CDNA-20UL Cell Line OriginHuman foetal lungCell Line DescriptionEstablished from foetal lung tissue. The cells have a finite lifespan and a similar virus susceptibility to the line MRC-5.ApplicationVirus studies: rhinovirusesCulture MediumEMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human lung (fetal) growth mode Adherent karyotype 2n = 46, diploid morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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MRC-9
Кат. номер 85020202-CDNA-20UL 85020202-DNA-5UG 85020202-RNA-5UG 85020202-1VL Cell Line OriginHuman Caucasian foetal lungCell Line DescriptionEstablished from normal lung tissue of a 15 week old female Caucasian foetus. Like MRC-5 the line is susceptible to a wide range of viruses. At least 40 population doublings expected.ApplicationVirus studies: rhinoviruses, production of viral vaccinesDNA ProfileSTR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 8,11
D16S539: 12
D5S818: 12,13
D7S820: 10,11
THO1: 6,8
TPOX: 11
vWA: 16,17Culture MediumEMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human lung (fetal) growth mode Adherent karyotype 2n = 46, diploid morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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MSV.B
Кат. номер 90031901-DNA-5UG 90031901-1VL 90031901-RNA-5UG Cell Line OriginMouse BALB/c embryonic fibroblast, M-MSV transformedCell Line DescriptionDerived by transformation of embryonic BALB/c fibroblasts with M-MSV. The cells have been used as a control for cells treated with interferon.ApplicationInterferon, transformation and virus studies: M-MSVCulture MediumRPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse embryo growth mode Adherent karyotype Not specified morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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MSVIF-TK+
Кат. номер 90031902-1VL 90031902-DNA-5UG 90031902-RNA-5UG Cell Line OriginMouse BALB/c embryonic fibroblast, M-MSV transformedCell Line DescriptionDerived from the MSV.B line (Sigma Catalogue number. 90031901) which has been exposed to 50 IU/ml Interferon for over 200 passages. Unlike the parent line, the cells are non-malignant and do not form tumours in nude mice.ApplicationInterferon, transformation and virus studies: M-MSVCulture MediumEMEM (EBSS) + 1% Non Essential Amino Acids (NEAA) or RPMI 1640 + 2mM Glutamine + 50-100 IU/ml murine IFN + 5-10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse embryo growth mode Adherent karyotype Not specified morphology Fibroblast products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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MT14B human cell line
Кат. номер 88052098-1VL 88052098-CDNA-20UL 88052098-DNA-5UG 88052098-RNA-5UG General descriptionConsanguineous: YesCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 3101
Cw: 304
B: 4001
HLA DRB1: 404
HLA DRB4: 101
HLA DQAP: 3.1
HLA DQA1: 3
HLA DQB1: 302
HLA DPB1: 402Serological ProfileHLA A: 31
HLA A ief: 31
HLA C: 10
HLA B: 60
HLA B ief: 60
Bw4/bw6: 6
HLA Dw: 14
HLA DR: 4
HLA DR53: 53
HLA DQ: 8Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Australian Caucasian gender male growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
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MT3/B4
Кат. номер 92030607-DNA-5UG 92030607-1VL 92030607-RNA-5UG Cell Line OriginNot specifiedCell Line DescriptionThis monoclonal antibody showed strong neutralising activity against homologous IFN alpha-2. Cross-reactions with some h IFN alpha subtypes have been detected but no cross reaction with h IFN beta or gamma was shown.ImmunogenRecombinant human interferon alpha-2ApplicationImmuno assays, neutralisation of IFN alpha-2 activity.Culture MediumRPMI 1640 or DMEM + 2 mM glutamine + 7% Fetal Bovine Serum (FBS) (heat inactivated)Subculture RoutineMaintain cultures between 1-5x100,000 cells/ml; 5% CO2; 37 °C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma P3X63Ag8 Reactivity Anti-rh interferon alpha-2 (recombinant human) and related subtypes application(s) cell culture | mammalian: suitable isotype IgG1 shipped in dry ice storage temp. −196°C
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MT4/E4
Кат. номер 92030608-RNA-5UG 92030608-1VL 92030608-DNA-5UG Cell Line OriginMouse P3-X63-Ag8 x Mouse BALB/c spleenCell Line DescriptionMT4/E4 is a very strong neutralising monoclonal antibody with a wide range of neutralising monoclonal activities against homologous human IFN-α-2. The heterologous IFN-α-1 is weakly bound but also shows cross-reactive neutralisation. There is no cross-reaction with human IFN- or IFN-gamma.ImmunogenRecombinant human interferon alpha-2ApplicationCytokine assays, studies of anti-viral activity of interferon, neutralisation of interferon-α-2 activity.Culture MediumRPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS) (heat inactivated)Subculture RoutineMaintain cultures between 5-9x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma P3X63Ag8 Reactivity Anti-Interferon α-2, human and some related subtypes application(s) cell culture | mammalian: suitable isotype IgG1 shipped in dry ice storage temp. −196°C
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MTSV1-7 CE1
Кат. номер 10081202-DNA-5UG 10081202-CDNA-20UL 10081202-1VL 10081202-RNA-5UG Cell Line OriginBreast cancer, morphogenesis, adhesion propertiesCell Line DescriptionMTSV1-7 CE1 is a clonal derivative of the human mammary luminal epithelial cell line MTSV1-7 transfected with ERBB2 expression vector, enabling the over-expression of ERBB2 proto-oncogene. Cultures of MTSV1-7 were transfected with the pSV2-erbB2 construct together with the pSV2neo plasmid. (See also 1-7HB2 Sigma catalog number 10081201).
The MTSV1-7 CE1 cell line is an ideal tool for investigation of the role of the ERBB2 receptor proto-oncogene in breast cancer morphogenesis and adhesion. MTSV1-7 CE1 cells over-express the ERBB2 receptor, inducing a reduced ability to undergo morphogenesis in vitro, and reduced expression of surface adhesion molecules E-Cadherin and alpha-2 integrin. Inhibition of morphogenesis and transcription of adhesion molecules in human mammary epithelial cells can be affected by signals generated by the ERBB2 receptor, suggesting a role for ERBB2 over-expression in tumour progression and metastasis.DNA ProfileSTR-PCR Data:
Amelogenin: X
CSF1PO: 12,13
D13S317: 12
D16S539: 8,13
D5S818: 10,13
D7S820: 8,10
THO1: 7,9
TPOX: 11
vWA: 14,17Culture MediumDMEM + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS) + Hydrocortisone (5 μg/ml) + Bovine Insulin (10 μg/ml).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 3 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO<sub>2</sub>; 37 °C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source human breast growth mode Adherent karyotype Not specified morphology Cuboidal morphology at confluence products Formation of disorganised structures in collagen type I gels. receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice
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Mv.1.Lu
Кат. номер 88050503-DNA-5UG 88050503-RNA-5UG 88050503-1VL Cell Line OriginMink lungCell Line DescriptionDerived from trypsinised lungs of several foetuses of Aleutian mink. Used for focus forming assays for murine and feline sarcoma viruses.ApplicationVirus focus forming assays: MSV, feline sarcoma virusCulture MediumEMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mink lung growth mode Adherent karyotype 2n = 30; diploid and pseudodiploid morphology Epithelial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable shipped in dry ice storage temp. −196°C
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My-La CD4+
Кат. номер 95051032-DNA-5UG 95051032-CDNA-20UL 95051032-1VL 95051032-RNA-5UG Cell Line OriginHuman Caucasian CD4+ T Lymphocyte, mycosis fungoidesCell Line DescriptionTwo different continuous cell lines were established from a plaque biopsy of an 82-year old male with mycosis fungoides stage II by inclusion of IL-2 and IL-4 in the culture medium: My-La, CD4+, (referred to in the literature as My-La, marker chromosome) and My-La, CD8+ (referred to in the literature as My-La 46,XY-18+i(18q) (Sigma Catalogue number. 95051033). My-La CD4+ is strictly dependent on exogenous IL-2 and IL-4 in the culture medium whereas My-La CD8+ on IL-2 only. Both show characteristic chromosome markers and are double homozygous for HLA-A1.B8. My-La CD8+ expresses β-13 of the T cell receptor and phenotypically weak staining was observed for CD2, CD3, CD8, CD25, CD71 and TCR-1 α / . My-La CD4+ expresses β-18 of the T cell receptor plus staining for CD2, CD3, CD4,CD25, CD71, HLA-DR and TCR-1 α/ . There has been no evidence of HTLV-I infection in the cell lines and serum from the patient did not demonstrate antibodies against HTLV-I and HTLV-II. Both cell lines show a low viability after resuscitation from frozen and a long lag time phase until exponential growth is reached.ApplicationStudy of antigen-independent proliferation of T- cell lyphoma from mycosis fungoides patient.DNA ProfileSTR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,12
D16S539: 8,11
D5S818: 9,12
D7S820: 10,11
THO1: 6
TPOX: 8,12
vWA: 17Culture MediumRPMI 1640 (R0883), + 2mM L-Glutamine (G7513), + 10 U/ml IL-2 (H7041), + 10 U/ml IL-4 (H7291), + 10% Human AB Serum (H4522),Subculture RoutineMaintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37°C. Split only 1:2 as maximum dilution: freeze in 45% growth medium, 45% human AB serum plus 10% DMSO, low viability on resuscitation. Use of human serum produces high levels of debris in cultures. Population doubling time 20-24 hours.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human lymph growth mode Suspension karyotype Not specified morphology Lymphoblastoid products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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My-La CD8+
Кат. номер 95051033-CDNA-20UL 95051033-RNA-5UG 95051033-DNA-5UG 95051033-1VL Cell Line OriginHuman Caucasian T Lymphocyte, mycosis fungoides.Cell Line DescriptionThe cell line My-La, CD8+, also known as My-La, marker chromosome was established from a plaque biopsy of an 82 year old Caucasian patient with mycosis fungoides stage II. Together with My-La, CD8+ a second cell line has been established (My-La, CD4+ Sigma Catalogue number. 95051032) by including IL-2 and IL-4 in the culture medium. My-La, CD8+ shows characteristic chromosome markers, express -13 of the T-cell receptor and are double homozygous for HLA-A1.B8. Phenotypically a weak staining was observed for CD2, CD3, CD8, CD25, CD71 and TCR-1 α / . There has been no evidence of HTLV-I infection in the cell line and serum from the patient did not demonstrate antibodies against HTLV-I and HTLV-II. My-La, CD8+ cells show a low viability after resuscitation from frozen and a long lag time phase until exponential growth is reached.ApplicationStudy of antigen-independent proliferation of T- cell lyphoma from mycosis fungoides patient.Culture MediumRPMI 1640 + 2mM Glutamine + 100 u/ml IL-2 + 10% human AB seumSubculture RoutineMaintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37°C. Split only 1:2 as maximum dilution: freeze in growth medium plus 10% DMSO, low viability on resuscitation. Use of human serum produces high levels of debris in cultures.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human lymph growth mode Suspension karyotype Not specified morphology Lymphoblastoid products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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Myb2-32
Кат. номер 84101003-RNA-5UG 84101003-1VL 84101003-DNA-5UG Cell Line OriginMouse Sp2/0 x Mouse BALB/c spleenCell Line DescriptionThe antibody recognises an antigen on the primary structure of bp37v-myb and binds at or near same site as Myb2-2 and Myb2-3. It cross reacts with v-myb gene product of 45kDa and c-myb gene product of 75kDa. The antibody recognises E26 oncogene product p135gag-myb-ets.ImmunogenThe bacterially expressed fusion protein, bp37v-mybCulture MediumRPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma Sp2/0 Reactivity Anti-myb gene products, avian application(s) cell culture | mammalian: suitable isotype IgG1, kappa shipped in dry ice storage temp. −196°C
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Myb2-37
Кат. номер 84110802-RNA-5UG 84110802-1VL 84110802-DNA-5UG Cell Line OriginMouse Sp2/0 x Mouse BALB/c spleenCell Line DescriptionThe antibody recognises an antigen on the primary structure of bp37v-myb and cross reacts with c-myb gene product of 45kDa and v-myb gene product of 75kDa. It recognises a highly conserved normal cellular protein of 37kDa localised mainly in nucleus.ImmunogenThe bacterially expressed fusion protein, bp37v-mybCulture MediumRPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma Sp2/0 Reactivity Anti-myb gene products, avian application(s) cell culture | mammalian: suitable isotype IgG2b, kappa shipped in dry ice storage temp. −196°C
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Myb2-7
Кат. номер 84110801-RNA-5UG 84110801-DNA-5UG 84110801-1VL Cell Line OriginMouse Sp2/0 x Mouse BALB/c spleenCell Line DescriptionThe antibody recognises an antigen on the primary structure of bp37v-myb and cross reacts with v-myb gene product of 45kDa and c-myb gene product of 75kDa. The antibody recognises E26 oncogene product p135gag-myb-ets.ImmunogenThe bacterially expressed fusion protein, bp37v-mybCulture MediumRPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma Sp2/0 Reactivity Anti-myb gene products, avian application(s) cell culture | mammalian: suitable isotype IgG1, kappa shipped in dry ice storage temp. −196°C
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Mycl-3C7
Кат. номер 85102203-DNA-5UG 85102203-RNA-5UG 85102203-1VL Cell Line OriginMouse Sp2/0 x Mouse BALB/c spleenCell Line DescriptionThe antibody recognises the human p62c-myc gene product. It is specific for the peptide D sequence and cross reacts with the p64/66 gene product of the mouse c-myc gene.ImmunogenA synthetic peptide, corresponding to residues 171-188 (Peptide D) of human p62c-myc sequence, conjugated to KLHCulture MediumRPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma Sp2/0 Reactivity Anti-c-Myc gene product, human application(s) cell culture | mammalian: suitable isotype IgG1, kappa shipped in dry ice storage temp. −196°C
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Mycl-8F9
Кат. номер 85102204-1VL 85102204-DNA-5UG 85102204-RNA-5UG Cell Line OriginMouse Sp2/0 x Mouse BALB/c spleenCell Line DescriptionThe antibody recognises the human p62c-myc gene product. It is specific for the peptide D sequence and cross reacts with the p64/66 gene product of the mouse c-myc gene.ImmunogenA synthetic peptide, corresponding to residues 171-188 (Peptide D) of human p62c-myc sequence, conjugated to KLHCulture MediumRPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma Sp2/0 Reactivity Anti-c-Myc gene product, human application(s) cell culture | mammalian: suitable isotype IgG1, kappa shipped in dry ice storage temp. −196°C
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Mycl-9E10
Кат. номер 85102202-RNA-5UG 85102202-1VL 85102202-DNA-5UG Cell Line OriginMouse Sp2/0 x Mouse BALB/c spleenCell Line DescriptionThe antibody recognises human p62c-myc gene product and is specific for C-terminal peptide G. The antibody failed to immunoprecipitate p62 c-myc, but recognised the imunoblotted protein. Compared to other hybridomas specific to this antigen (Mycl-8F9, Mycl-3C7, CT9-B7, CT14-B2) Mycl-9E10 is the most sensitive probe for immunoblots. Conformation of antigen is critical for antibody recognition. No reported cross-reaction, with chicken p110gag-myc protein or mouse c-myc protein. This cell line is available as a growing culture only to customers in Europe.ImmunogenA synthetic peptide, corresponding to residues 408-432(C-terminus of Peptide G) of human p62c-myc sequence, conjugated to KLHCulture MediumRPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cells are slow to grow. This cell line is available as a growing culture only to customers in Europe.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma Sp2/0 Reactivity Anti-c-Myc gene product, human application(s) cell culture | mammalian: suitable isotype IgG1, kappa shipped in dry ice storage temp. −196°C
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MZ070782 human cell line
Кат. номер 88052002-DNA-5UG 88052002-RNA-5UG 88052002-CDNA-20UL 88052002-1VL General descriptionConsanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileA: 2402
Cw: 02022 0802
B: 1402
HLA DRA: 101
HLA DRB1: 102
HLA DRB6: 101
HLA DQAP: 1.1
HLA DQA1: 101
HLA DQB1: 501
HLA DPA1: 1
HLA DPB1: 401Serological ProfileHLA A: 24
HLA A ief: 24.1
HLA C: 2 8
HLA B: 14
HLA B ief: 14
Bw4/bw6: 6
HLA Dw: 20
HLA DR: 1
HLA DQ: 5
HLA DP: 4Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Ashkenasi Jewish gender male growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
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N1-S1
Кат. номер 90011902-RNA-5UG 90011902-DNA-5UG 90011902-1VL Cell Line OriginRat hepatomaCell Line DescriptionEstablished from the ascitic fluid of a Novikoff hepatoma. The cells can be synchronised for cell cycle studies. N1-S1 has also been used for studies of pyrimidine metabolism, DNA synthesis and methylation and ribosomal RNA maturation.ApplicationCell cycle, metabolic and mutation studiesCulture MediumSwimm′s S-77 + 2mM Glutamine + 0.1% Pluronic F68 +10% Foetal Bovine Serum (FBS).Subculture RoutineMaintain cultures between 4x10,000 to 1,000,000 cells/ml; 5% CO2; 37°C. Subculture every 3-4 days by centrifugation or medium addition.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat liver growth mode Adherent karyotype Not specified morphology Epithelial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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N115-BU-1
Кат. номер 8062547-DNA-5UG 8062547-1VL 8062547-RNA-5UG Cell Line OriginMouse neuroblastoma, bromodeoxyuridine resistantCell Line DescriptionA bromodeoxyuridine-resistant mutant of N1E-115 (Sigma Catalogue number 08062511). The cell line N1E-115 is an adrenergic cell line derived from mouse neuroblastoma C1300 (Mouse strain A/Jax)ApplicationUsed in the generation of cell hybrids.Culture MediumDMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 1 mM 8-bromodeoxyuridine. The cells can be grown in the absence of bromodeoxyuridine.Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse nerve growth mode Adherent karyotype Not specified morphology Not specified products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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N115-BU-10
Кат. номер 8062559-RNA-5UG 8062559-DNA-5UG 8062559-1VL Cell Line OriginMouse NeuroblastomaCell Line DescriptionA bromodeoxyuridine-resistant mutant of N1E-115 (Sigma Catalogue number 08062511). The cell line N1E-115 is an adrenergic cell line derived from mouse neuroblastoma C1300 (Mouse strain A/Jax)ApplicationUsed in the generation of cell hybrids.Culture MediumDMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 1 mM 8-bromodeoxyuridine. The cells can be grown in the absence of bromodeoxyuridineSubculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse nerve growth mode Adherent karyotype Not specified morphology Neuronal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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N115-BU-2
Кат. номер 8062552-RNA-5UG 8062552-1VL 8062552-DNA-5UG Cell Line OriginMouse neuroblastoma, bromodeoxyuridine resistantCell Line DescriptionA bromodeoxyuridine-resistant mutant of N1E-115 (Sigma Catalogue number 08062511). The cell line N1E-115 is an adrenergic cell line derived from mouse neuroblastoma C1300 (Mouse strain A/Jax)ApplicationUsed in the generation of cell hybrids.Culture MediumDMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 1 mM 8-bromodeoxyuridine. The cells can be grown in the absence of bromodeoxyuridine.Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm? using 0.05% trypsin/EDTA; 5% CO2; 37?C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse nerve growth mode Adherent karyotype Not specified morphology Neuronal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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N115-BU-7
Кат. номер 8062554-RNA-5UG 8062554-1VL 8062554-DNA-5UG Cell Line OriginMouse NeuroblastomaCell Line DescriptionA bromodeoxyuridine-resistant mutant of N1E-115 (Sigma Catalogue number 08062511). The cell line N1E-115 is an adrenergic cell line derived from mouse neuroblastoma C1300 (Mouse strain A/Jax)ApplicationUsed in the generation of cell hybrids.Culture MediumDMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 1 mM 8-bromodeoxyuridine. The cells can be grown in the absence of bromodeoxyuridineSubculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse nerve growth mode Adherent karyotype Not specified morphology Neuronal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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N115-BU-8
Кат. номер 8062519-1VL 8062519-RNA-5UG 8062519-DNA-5UG Cell Line OriginMouse NeuroblastomaCell Line DescriptionA bromodeoxyuridine-resistant mutant of N1E-115 (Sigma Catalogue number 08062511). The cell line N1E-115 is an adrenergic cell line derived from mouse neuroblastoma C1300 (Mouse strain A/Jax)ApplicationUsed in the generation of cell hybrids.Culture MediumDMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 1 mM 8-bromodeoxyuridine. The cells can be grown in the absence of bromodeoxyuridineSubculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse nerve growth mode Adherent karyotype Not specified morphology Neuronal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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N115-BU-9
Кат. номер 8062558-RNA-5UG 8062558-DNA-5UG 8062558-1VL Cell Line OriginMouse NeuroblastomaCell Line DescriptionA bromodeoxyuridine-resistant mutant of N1E-115 (Sigma Catalogue number 08062511). The cell line N1E-115 is an adrenergic cell line derived from mouse neuroblastoma C1300 (Mouse strain A/Jax).ApplicationUsed in the generation of cell hybrids.Culture MediumDMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 1 mM 8-bromodeoxyuridine. The cells can be grown in the absence of bromodeoxyuridineSubculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse nerve growth mode Adherent karyotype Not specified morphology Neuronal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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N18
Кат. номер 88112301-DNA-5UG 88112301-1VL 88112301-RNA-5UG Cell Line OriginMouse neuroblastoma x Rat glioma hybridCell Line DescriptionNeuroblastoma x glioma hybrid was formed by Sendai virus-induced fusion of the mouse neuroblastoma clone N18.ApplicationHybridisation, fusion partner and virus studies: Sendai virusCulture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source Mouse x Rat Hybridoma hybrid growth mode Adherent karyotype Not specified morphology Neuronal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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N18 (Hamprecht)
Кат. номер 8062527-1VL 8062527-DNA-5UG 8062527-RNA-5UG Cell Line OriginMouse neuroblastomaCell Line DescriptionN18 is a clonal line produced from the mouse neuroblastoma C1300. (Mouse strain A/Jax). ECACC has two catalogue entries for the N18 cell line which differ in that they were deposited by different groups: N18 from the original group that derived the cell line (Sigma Catalogue number 88112301) and N18(Hamprecht) (Sigma Catalogue number. 08062527). Several other cell lines in the ECACC catalogue have been derived from N18(Hamprecht).ApplicationCan be used as a neuronal modelCulture MediumDMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS)Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse nerve growth mode Adherent karyotype Not specified morphology Not specified products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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N18TG2
Кат. номер 8062523-1VL 8062523-RNA-5UG 8062523-DNA-5UG Cell Line OriginMouse neuroblastomaCell Line DescriptionA 6-thioguanine-resistant clone of N18 (a clonal line produced from the mouse neuroblastoma C1300). (Mouse strain A/Jax). This cell line exhibits high levels of acetylcholinesterase. Found to be tumourigenic in BALB/c nude mice.ApplicationUsed in the generation of cell hybrids.Culture MediumDMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 0.1 mM 6-thioguanineSubculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse nerve growth mode Adherent karyotype mean chromosome number is 89 morphology Neuronal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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N1E-115
Кат. номер 88112303-RNA-5UG 88112303-1VL 88112303-DNA-5UG Cell Line OriginMouse neuroblastomaCell Line DescriptionAdrenergic clone derived from the mouse neuroblastoma C1300 tumour.ApplicationTumourigenicity studiesCulture MediumDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 ; 5% CO2; 37°C. Cells only attach loosely to the flask and can be detatched by gently tapping the flask. The cells can completely detatch during shipment. If this occuOther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse nerve growth mode Semi-adherent karyotype Not specified morphology Neuronal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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N1E-115-1
Кат. номер 8062511-RNA-5UG 8062511-DNA-5UG 8062511-1VL Cell Line OriginMouse neuroblastomaCell Line DescriptionA subclone of the cell line N1E-115 established by Amano et al. The cell line N1E-115 is an adrenergic cell line derived from mouse neuroblastoma C1300. (Mouse strain A/Jax). N1E-115 cells are reported to be slightly contaminated with with a cell type of the morphology of C6 glioma cells. It is recommended to use N1E-115-1, a subclone of N1E-115, that is devoid of the contamination.Applicationcan be used as a neuronal modelCulture MediumDMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS)Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C. Population doubling approximately 20 hours.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse nerve growth mode Adherent karyotype modal no. of chromosomes is 192 see reference morphology neuronal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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N2102Ep Clone 2/A6
Кат. номер 6011803-DNA-5UG 6011803-CDNA-20UL 6011803-1VL 6011803-RNA-5UG Cell Line OriginHuman embryonal carcinomaCell Line DescriptionA non seminomatous germ cell carcinoma derived cell line. This cell line was established from a single cell clone from a subline of cell line 2102Ep passaged through a nude mouse. This cell line is tumourigenic in nude mice.Culture MediumDMEM (High Glucose) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). No sodium pyruvate.Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at >6x10,000 cells/cm2 using 0.25% trypsin or trypsin or trypsin/EDTA; 10% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human embryo growth mode Adherent karyotype Not specified morphology Not specified products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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N4TG3
Кат. номер 8062515-1VL 8062515-DNA-5UG 8062515-RNA-5UG Cell Line OriginMouse neuroblastomaCell Line DescriptionA 6-thioguanine-resistant clone of N4 (a clonal line produced from the mouse neuroblastoma C1300). (Mouse strain A/Jax). This cell line lacks any appreciable activity of either choline acetyltransferase or tyrosine hydroxylase enzymes.ApplicationUsed in the generation of cell hybrids.Culture MediumDMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 0.1 mM 6-thioguanineSubculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse nerve growth mode Adherent karyotype Modal No of chromosomes is 86 morphology Neuronal products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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NA1/34.HLK
Кат. номер 85102243-1VL 85102243-RNA-5UG 85102243-DNA-5UG Cell Line OriginMouse NS1 x Mouse BALB/c spleenCell Line DescriptionThe antibody detects the CD1a antigen on human cortical thymocytes, Langerhans cells and interdigitating cells.ImmunogenHuman thymocytesCulture MediumDMEM + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS)Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.Параметрыbiological source Mouse x Mouse Hybridoma ImmunoDon BALB/c mouse spleen Myeloma NS1 Reactivity Anti-CD1a, human application(s) cell culture | mammalian: suitable isotype IgG2a shipped in dry ice storage temp. −196°C
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NAI 006 human cell line
Кат. номер 94082272-DNA-5UG 94082272-CDNA-20UL 94082272-RNA-5UG 94082272-1VL General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileHLA DRB1: 0405 09
HLA DRB4: 101
HLA DQA1: 03 0302
HLA DQB1: 0303 0401
HLA DPB1: 02012 0402Serological ProfileHLA A: 2 26
HLA A ief: 2.2
HLA C: 1
HLA B: 35 7Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Not specified gender not specified growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
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NAI 026 human cell line
Кат. номер 94082271-RNA-5UG 94082271-CDNA-20UL 94082271-DNA-5UG 94082271-1VL General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileHLA DRB1: 405
HLA DRB4: 101
HLA DQAP: 3.1
HLA DQA1: 3
HLA DQB1: 401
HLA DPB1: 02012 0301Serological ProfileHLA A: 24 26
HLA A ief: 24.1
HLA C: 1 3
HLA B: 35 59
HLA B ief: 35.2
Bw4/bw6: 6Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Not specified gender not specified growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
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NAI 031 human cell line
Кат. номер 94082273-RNA-5UG 94082273-DNA-5UG 94082273-CDNA-20UL 94082273-1VL General descriptionHomozygous: No
Consanguineous: NoCell Line DescriptionThis cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.DNA ProfileHLA DRB1: 0405 0803
HLA DRB4: 101
HLA DQAP: 1.4 3.1
HLA DQA1: 03 0103
HLA DQB1: 0401 0601
HLA DPB1: 02012 0402Serological ProfileHLA A: 24
HLA A ief: 24.1
HLA C: 1
HLA B: 54 59
Bw4/bw6: 4 6Culture MediumRPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.Subculture RoutineMaintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.DisclaimerThis cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.Параметрыbiological source human blood ethnicity Not specified gender not specified growth mode Suspension karyotype Not specified morphology Lymphoblastoid application(s) cell culture | mammalian: suitable shipped in dry ice
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Namalwa
Кат. номер 87060801-1VL 87060801-RNA-5UG 87060801-DNA-5UG 87060801-CDNA-20UL Cell Line OriginHuman Burkitt′s LymphomaCell Line DescriptionSecretes small amounts of an IgM monoclonal antibody of unknown specificity. It has been used for commercial production of human interferon. The cells contain the EBV genome and should be handled under laboratory containment level 2.ApplicationInterferon studiesDNA ProfileSTR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 11,12
D16S539: 9
D5S818: 12,13
D7S820: 11
THO1: 7,9.3
TPOX: 6,11
vWA: 14Culture MediumRPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineMaintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. A maximum density of 2x1,000,000 cells/ml is possible.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human blood growth mode Suspension karyotype Modal no. 44; hypodiploid morphology Lymphoblast products Immunoglobulin M (IgM), lambda receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C Узнать цену -
Nb2-11 Cell Line from rat
eCl@ss: 32190102
Кат. номер 97041101-RNA-5UG 97041101-1VL 97041101-DNA-5UG Cell Line OriginRat lymphomaCell Line DescriptionNb2-11 is a clone of the Nb-2 rat lymphoma line which was derived from a transplant of a lymphoma that developed in the thymus/lymph node of a male noble (Nb) strain rat following prolonged oestrogen treatment. The cells are of the pre-T cell origin and their proliferation is dependent on mammalian lactogens, such as prolactin. Nb2-11 can also be mitogenically stimulated by IL-2. Injection of Nb2 cells into Nb rats gives rise to malignant tumours that are highly sensitive to treatment with vinca alkaloids. Karyotypic analysis has shown that the cell line has only five well developed chromosome abnormalities. The cells do not express surface immunoglobulin, and their lactogen dependency was confirmed before deposit. Protocols for the use of Nb2-11 cells in bioassays are available from ECACC on request.Application1) in vitro bioassay of lactogenic hormones.
2) Studies of the mechanisms of lactogen induced mitogenesis.
3) Studies into the action of vinca alkaloids.
4) Studies of tumour progression.Culture MediumFischer′s medium + 10% Fotal Bovine Serum (FBS) + 10% Horse Serum (HS) (gelding) + 0.075% Sodium Bicarbonate + 0.05 mM 2-Mercaptoethanol (2ME) + 2 mM Glutamine.Subculture RoutineAt resuscitation seed cultures at 3-5 x 100,000 cells/ml. Once established cultures should be seeded at 4 x 1,000 and 12 x 1,000 cells/ml for culture periods of 96 and 72 hours respectively. Do not exceed 9 x 100,000 cells/ml. Avoid using dilutions of cells with fresh medium at greater than 1:10 ratios; culture doubling time 12 hours.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat lymph growth mode Suspension karyotype 42XY,-17,t(2,4)(q22,q24),t(10,11)(q12,q12),+der(15)t(15,?)(p12,?),-18,+der(18)t(18,?)(p12,?) morphology Lymphoblast products Not specified receptors Modified prolactin receptor (the Nb2 form) application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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NB4 1A3
Кат. номер 89121405-DNA-5UG 89121405-1VL 89121405-RNA-5UG Cell Line OriginMouse C-1300 NeuroblastomaCell Line DescriptionCloned from the C1300 mouse neuroblastoma line. Adapted to culture by alternate in vivo and in vitro passage. Cells have characteristics of mature neurons and secrete the enzymes for the synthesis of the neurotransmitters, choline acetylase and tyrosine hydroxylase. Cells are susceptible to VSV, HSV and Vaccinia viruses.ApplicationTumourigenicity, enzymatic and virus studies: VSV, HSV, vaccinia virusCulture MediumHam′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source mouse nerve growth mode Adherent karyotype Unstable morphology Neuroblast products Acetylcholinesterase, choline acetylase, tyrosine hydroxylase receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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NB69
Кат. номер 99072802-DNA-5UG 99072802-1VL 99072802-RNA-5UG 99072802-CDNA-20UL Cell Line OriginHuman neuroblastoma (Stage III)Cell Line DescriptionSample was isolated from a 16 month old maleDNA ProfileSTR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8
D16S539: 9,11
D5S818: 11,12
D7S820: 10
THO1: 8
TPOX: 11
vWA: 17,18Culture MediumRPMI 1640 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).Subculture RoutineCells can grow in aggregates as well as attached. Split sub-confluent cultures 1:4 using 0.25% trypsOther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human brain growth mode Semi-adherent karyotype Not specified morphology Not specified products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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NBT-II
Кат. номер 93012901-1VL 93012901-DNA-5UG 93012901-RNA-5UG Cell Line OriginRat Wistar bladder tumourCell Line DescriptionDerived from a male Wistar rat bladder tumour induced with N-butyl-N-(4-hydroxybutyl) nitrosamine. The histotypic differentiation normally displayed by these cells is inhibited by vitamin A and gives rise to the appearance of giant cells.ApplicationMaturation of epidermoid carcinomaCulture MediumEMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2.5x1,000,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source rat bladder growth mode Adherent karyotype Not specified morphology Epithelial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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NCI-H292
Кат. номер 91091815-DNA-5UG 91091815-CDNA-20UL 91091815-RNA-5UG 91091815-1VL Cell Line OriginHuman lung, mucoepidermoid carcinomaCell Line DescriptionDerived from a cervical node metastasis of a pulmonary mucoepidermoid carcinoma from a 32 year old female. Cells retain mucoepidermoid characteristics in culture and support replication of Hepatitis B virus. Some of the cells contain numerous small mucin-containing granules and are L-DOPA decaroxylase negative. This cell line has been selected as a prototype for transfecting subgenome fragments into human cells for studying the role of HBV and its individual genes in the pathogenesis of viral hepatitis and liver cancer. The cells are mucicarmine positive and also stain positively for keratin and vimentin but negative for neurofilament triplet protein. Ethnicity: Black.ApplicationNCI-H292 has been used to determine the ability of the cell line to support the replication of mumps virus.DNA ProfileSTR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 11,12
D16S539: 9,13
D5S818: 13
D7S820: 10
THO1: 8
TPOX: 8,11
vWA: 16,17Culture MediumRPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human lung growth mode Adherent karyotype Modal no. 47 morphology Epithelial products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) metastasis shipped in dry ice storage temp. −196°C
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NCI-H322 Cell Line human
Кат. номер 95111734-DNA-5UG 95111734-CDNA-20UL 95111734-RNA-5UG 95111734-1VL Cell Line OriginHuman Caucasian bronchioalveolar carcinomaCell Line DescriptionNCI-H322 was derived in 1981 from a primary bronchioalveolar carcinoma of the lung from a 52 year old male taken prior to treatment. Ultrastructural studies of this non-small cell carcinoma cell line demonstrated the presence of cytoplasmic structures characteristic of Clara cells. NCI-H322 do not express UDP-glucuronosyltransferase, but are reported to express glutathione-S-transferase and phenol sulfotransferase. Expression of protein and RNA of SP-A, the major surfactant-associated protein was detected. The cells produce tumours in athymic mice.ApplicationTest system for evaluating cytotoxicity and genotoxicity of chemicals to human lung
NCI-H322 has been used to study the preclinical antitumor efficacy of BAY 1129980, which is an anti-C4.4A (Ly6/PLAUR domain-containing protein 3 precursor-LYPD3) antibody–drug conjugate for the treatment of non–small cell lung cancer.Culture MediumRPMI 1640 + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human lung (cervical node metastasis) description Human Caucasian bronchioalveolar carcinoma growth mode Adherent karyotype Not specified morphology Not specified products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) metastasis shipped in dry ice storage temp. −196°C
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NCI-H358
Кат. номер 95111733-DNA-5UG 95111733-1VL 95111733-RNA-5UG 95111733-CDNA-20UL Cell Line OriginHuman Caucasian bronchioalveolar carcinomaCell Line DescriptionNCI-H358 was isolated from a primary bronchioalveolar carcinoma of the lung from a Caucasian male taken prior to treatment. Ultrastructural studies of this non-small cell carcinoma of the lung (NSCLC) demonstrated the presence of granules characteristic of Clara cells. NCI-H358 do not express UDP-glucuronosyltransferases, but do express glutathione-S-transferase and phenol sulphotransferase. Expression of SP-A protein and RNA, the major surfactant-associated protein was detected. SP-B and SP-C RNA was not expressed. A complete homozygous deletion of the p53 gene and therefore a lack of p53 protein has been reported. A colony forming efficiency of 0.83% in soft agarose, and growth in serum-free media has been reported. The cells are tumourigenic in athymic nude mice, and exhibit a doubling time of 38 hours in RPMI 1640 medium. Since these cells are proficient in oxidation of xenobiotics but deficient in their conjugation with glucuronic acid they present a tool for analysing the role of glucuronic acid conjugation in the inactivation of chemicals in intact cells.ApplicationTest system for evaluating cytotoxicity and genotoxicity of chemicals to human lungDNA ProfileSTR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 12,13
D5S818: 10,12
D7S820: 10,11
THO1: 6
TPOX: 8,9
vWA: 17Culture MediumRPMI 1640 + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human lung (bronchoalveolar) growth mode Adherent karyotype Not specified morphology Not specified products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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NCI-H510A
Кат. номер 96020944-RNA-5UG 96020944-CDNA-20UL 96020944-1VL 96020944-DNA-5UG Cell Line OriginHuman lung carcinoma, adrenal gland metastasisCell Line DescriptionThe NCI-H510A cell line was derived by D. Carney, A.F. Gazdar amd associates in 1982 from an adrenal metastasis in an adult, 56-year-old, male Caucasian patient. The cells produce easily detectable p53 mRNA at levels comparable to those in normal lung tissue. The line does not exhibit any gross structural DNA abnormalities. The cells express elevated levels of four biochemical markers of small cell lung carcinoma (SCLC) i.e. neuron specific enolase, the brain isoenzyme creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity. This cell line is tumourigenic in nude mice (the cells form transplantable tumours with typical small cell carcinoma histology).Culture MediumRPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Subculture RoutineSplit sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 2-4x10,000 cells/cm2; 5% CO2; 37°C. This cell line grows as a mixture of cells in suspension and adherent cells. Subcultures can be prepared by scraping the adherent cells into the medium,Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human lung (adrenal metastasis) growth mode Adherent/aggregates in suspension karyotype Hypotriploid; modal number = 54; range = 46 to 57. Twenty to 25 marker chromosomes common to all cells. These include t(13q21q), der(1)t(1;21)(p36.1;q11), der(7)t(7;7)(p22;q22), 11p+, 12p+, and 15p+. morphology Not specified products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) metastasis shipped in dry ice storage temp. −196°C
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NCI-H69/CPR
Кат. номер 96042328-DNA-5UG 96042328-1VL 96042328-CDNA-20UL 96042328-RNA-5UG Cell Line OriginHuman small cell lung cancer, drug-resistantCell Line DescriptionNCI-H69/CPR is a drug resistant subline of NCI-H69 (Sigma Catalogue number. 91091802). Drug resistance was developed by addition of a stepwise increase in cisplatin to the growth medium of the parental line. The cell line exhibits a 5-fold resistance to cisplatin and is cross resistant to melphalan. A significant change in cellular glutathione content or sensitivity to cadmium chloride (as indicator of metallothionein content) was not detected, but changes in glutathione-S-transferase activity were seen. Cisplatin accumulation was unchanged compared with the parent line. It is recommended to culture the cells without drugs after resuscitation until the first passage.ApplicationDrug-resistance studies, small cell lung cancerCulture MediumRPMI 1640 + 2mM Glutamine + 0.4μg/ml cisplatin + 10% Foetal Bovine Serum (FBS).Subculture RoutineMaintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37C; resuscitate in drug-free medium until first passage.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human lung growth mode Aggregates in suspension karyotype Not specified morphology Not specified products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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NCI-H69/LX10
Кат. номер 96042331-RNA-5UG 96042331-1VL 96042331-DNA-5UG 96042331-CDNA-20UL Cell Line OriginHuman small cell lung cancer, drug-resistantCell Line DescriptionThe drug-resistant cell line NCI-H69/LX10 has been derived from the parent line NCI-H69 (ECACC No 91091802). It is recommended to culture the cells without drug upon resuscitation; add doxorubicine once growth is fully established.ApplicationDrug-resistance studiesCulture MediumRPMI 1640 + 2mM Glutamine + 1.0 μg/ml doxorubicine + 10% Foetal Bovine Serum (FBS).Subculture RoutineMaintain cultures between 3-9 x 100,000 cells/ml; 5% CO2, 37C; resuscitate in drug-free medium and add drug after growth has been established.Other NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human lung growth mode Aggregates in suspension karyotype Not specified morphology Not specified products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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NCI-H69/LX20
Кат. номер 96042332-CDNA-20UL 96042332-RNA-5UG 96042332-1VL 96042332-DNA-5UG Cell Line OriginHuman Caucasian small cell lung carcinomaCell Line DescriptionThe drug-resistant cell line NCI-H69/LX20 has been derived by exposure of the parent line, H69 (ECACC Number 91091802) to doxorubicin, also known as adriamycin. These multi drug resistant (MDR) cells have been found to be recognised by various anti-P-glycoprotein antibodies.ApplicationAnalysis of acquired drug resistance, study of small cell lung carcinoma, MDR studies.Culture MediumRPMI 1640 + 2mM Glutamine + 2μg/ml Doxorubicin + 10% Foetal Bovine Serum (FBS). ECACC has found that the following supplement will assist good growth of cells: sodium selenite 10nM, Insulin 250ng/ml, Transferrin 10ug/ml and sodium pyruvate 1mM.Subculture RoutineMaintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37°C. It is recommended to culture the cells without drug upon resuscitation until the first passage. Resuscitate into 9 ml of medium. Immediately after resuscitation perform a centrifugation steOther NotesAdditional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.Параметрыbiological source human lung growth mode Aggregates in suspension karyotype Not specified morphology Not specified products Not specified receptors Not specified application(s) cell culture | mammalian: suitable relevant disease(s) cancer shipped in dry ice storage temp. −196°C
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