Cell Lines &amp; Specialty Cell Culture

In form of tumorigenic ascites shows diminished expression of histocompatibility antigens and readily overcomes histoincompatibility barriers. Immunoresistant TA3/Ha sub-line arose spontaneously without subjection to any known immunological selection pressure.

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells grow partly in suspension, therefore spin down supernatant at time of subculture to collect the cells.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	mouse breast
growth mode	Adherent
karyotype	2n = 41
morphology	Epithelial
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

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TAI 034 human cell line

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

Cw: 802
HLA DRB1: 0102 1502
HLA DRB5: 102
HLA DQA1: 0101 0103
HLA DQB1: 0501 0601
HLA DPB1: 401

HLA A: 11 33
HLA C: 8
HLA B: 52 14
Bw4/bw6: 4 6

RPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.

biological source	human blood
ethnicity	Not specified
gender	not specified
growth mode	Suspension
karyotype	Not specified
morphology	Lymphoblastoid
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice

Human B cell lymphocyte, EBV transformed

RIDADR	NONH for all modes of transport
WGK Germany	3

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TAK3

Peripheral blood lymphocytes from a male Tunisian immortalised by EBV transformation. The cell line contains the following deletion, at the homozygous state, in the human immunoglobulin heavy chain constant region locus (IGH chromosome 14q32.3): deletion I (del G1-EP1-A1-GP-G2-G4) Gm-Am genotype Gm b; -; -A2m2/Gm b ; -; -A2m2. Gm allotypes are indicated in the order G3m; G1m; G2m. The absence of G1m and G2m allotypes due to the deletion of the IGHG1 and IGHG2 genes is indicated by (-).

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Maintain cultures at 5-9x100,000 cells/ml; 5% CO2; 37°C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	human blood
growth mode	Suspension
karyotype	Not specified
morphology	Lymphoblast
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

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TAT-1

Mouse x Mouse hybridoma

TAT-1 was developed by immunizing mice with extracted T. brucei brucei cytoskeleton and fusing the mouse spleen cells with the myeloma Sp2-0/Ag. This cell line has been cloned many times and the mixed subclass is now typical of the cloned line.

T. brucei brucei cytoskeleton (detergent extracted)

Detection of α tubulin.

DMEM (D6546), + 2mM L-Glutamine (G7513), + 10% FBS / FCS (F2442),

Maintain cultures between 1-9 x 100,000 cells/ml; 5% CO2; 37°C

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Mouse x Mouse Hybridoma
clone	TAT-1
ImmunoDon	Mouse spleen
Myeloma	Sp2-0/Ag
Reactivity	Anti-α-Tubulin
application(s)	cell culture \| mammalian: suitable
isotype	IgG12b + IgG2a
shipped in	dry ice
storage temp.	−196°C

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TB1 Lu (NBL-12)

Derived from the lung of an adult female bat. Rabies virus produces CPE in this cell line. The cells have been demonstrated to propagate for 20 more passages beyond passage 69.

Virus studies: rabies virus

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:8 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	bat lung
growth mode	Adherent
karyotype	2n = 48
morphology	Epithelial
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice
storage temp.	−196°C

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TF1 Cell Line human

Human erythroleukaemia

TF-1 have been derived from a patient with erythroleukaemia. They show complete growth dependency on GM-CSF or IL-3, and carry a homogeneous chromosomal abnormality (54,X). Constitutive expression of globin genes indicate commitment of TF-1 to the erythroid lineage, although glycophorin A or carbonyl anhydrase I are not expressed. On incubation with hemin or delta-aminolevulinic acid haemoglobin synthesis was induced, whereas TPA induced differentiation into macrophage-like cells. Erythroprotein also sustains short-term growth, but does not induce differentiation.

TF1 cell line has been used-

for tumorigenic studies
to study the effect of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3) on proliferation and TNFR2 expression in TF1 cells
in a cell-based proliferation assay to study the biological activities of anti-IL-6R Nanobody ALX-0061
to study the induction of STAT5 phosphorylation by IL-3

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 13
D13S317: 8,9
D16S539: 9,12
D5S818: 13
D7S820: 12
THO1: 7,9
TPOX: 8
vWA: 15,17

RPMI 1640 + 2mM Glutamine + 1% Sodium Pyruvate (NaP) + 2-5ng/ml Human Granulocyte Macrophage Colony Stimulating Factor (Human GM-CSF) + 10% Foetal Bovine Serum (FBS). It is recommended to add fresh GM-CSF every 48 hours.

Maintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37°C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	human blood
growth mode	Suspension
karyotype	Carries homogeneous chromosomal abnormality (54,X)
morphology	Not specified
products	Not specified
receptors	Interleukin 3 (IL-3) and Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

Mouse Sp2/5 x Mouse BALB/c spleen immunised with ricin chain

RIDADR	NONH for all modes of transport
WGK Germany	3

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TFT B1

Produces a monoclonal antibody (IgG1) which reacts with the chain of the plant toxin ricin.

Ricin chain

Has been used in a protocol for purifying the α chains (A1 and A2) of ricin with minimal cross-contamination for the preparation of immunotoxins.

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 100,000 - 1,000,000 cells/ml; 5% CO2; 37°C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Mouse x Mouse Hybridoma
ImmunoDon	BALB/c mouse spleen
Myeloma	Sp2/5 (clone of SP2/0 Ag-14)
Reactivity	Anti-Ricin β-chains
application(s)	cell culture \| mammalian: suitable
isotype	IgG1
shipped in	dry ice
storage temp.	−196°C

Mouse pancreatic acinar carcinoma

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TGP 49

TGP 49 has been isolated from an acinar cell carcinoma arising in the pancreas of a transgenic Tg(E1a-1-SV40E)Bri18 mouse. These mice carry a pseudogene construct composed of a pancreatic specific elastase-1 promoter linked to the SV40T antigen and thereby represent an animal model for pancreatic carcinogenesis. TGP 49 secretes low amounts of somatostatin, it is one of a series of cell lines isolated from pancreatic tumours deposited at ECACC. They have been reported not to secrete amylase or lipase but do secrete trypsin. Differentiation characteristics fluctuate from time to time in culture. Trabecular and clumps of small cells.

Study of endocrine pancreas carcinomas, hormone assays.

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	mouse pancreas
growth mode	Adherent
karyotype	Not specified
morphology	Trabecular
products	Somatostatin (low levels)
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

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TGP 52

Mouse islet cell tumour

TGP 52 has been isolated from an islet tumour arising in the pancreas of a transgenic Tg (E1a-1-SV40E) Bri18 mouse. These mice carry a pseudogene construct composed of a pancreatic specific elastase-1 promoter linked to the SV40T antigen and thereby represent an animal model for pancreatic carcinogenesis. TGP 52 cells are SV40T antigen positive and secrete somatostatin, it is one of a series of pancreatic tumour lines deposited at ECACC. Cells transiently produce insulin in culture followed by somatostatin.

Study of endocrine pancreas carcinomas, hormone assays.

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	mouse pancreas
growth mode	Adherent
karyotype	Not specified
morphology	Trabecular
products	Somatostatin
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

Mouse pancreatic islet cell tumour

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TGP 54

TGP 54 has been isolated from an islet tumour arising in the pancreas of a transgenic Tg (E1a-1-SV40E)Bri8 mouse. These mice carry a pseudogene construct composed of a pancreatic-specific elastase-1 promoter linked to the SV40T antigen and thereby represent an animal model for pancreatic carcinogenesis. TGP 54 cells are SV40T antigen positive and secrete low amounts of somatostatin, it is one of a series of cells obtained from pancreatic tumours deposited at ECACC.

Study of exocrine pancreas carcinomas, hormone secretion studies.

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	mouse pancreas
growth mode	Adherent
karyotype	Not specified
morphology	Not specified
products	Somatostatin
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

Mouse pancreatic small cell anaplastic carcinoma

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TGP 55

TGP 55 has been obtained from a small cell anaplastic carcinoma arising in the pancreas of a transgenic Tg(Ela-1SV40E)Bri 18 mouse these mice carry a pseudogene construct composed of a pancreatic-specific elastase-1 promoter linked to the coding region for SV40 T antigen and thereby represent an animal model for pancreatic carcinogenesis. TGP 55 cells are SV40T antigen positive and secrete somatostatin, it is one of a series of cell lines isolated from pancreatic tumours deposited at ECACC. Cells secrete somatostatin and insulin in culture. Adherent clumps of small cells.

Study of exocrine pancreas carcinomas, hormone secretion studies.

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	mouse pancreas
growth mode	Adherent
karyotype	Not specified
morphology	Polymorph
products	Somatostatin and Insulin
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

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THA human cell line

HLA DRB1: 1502 1308
HLA DRB3: 0202
HLA DRB5: 102
HLA DQB1: 05 06

HLA A: 3 31
HLA C: 4 ?7
HLA B: 35 70
HLA DR: 15 13
HLA DR52: 52
HLA DQ: 6

RPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.

biological source	human blood
ethnicity	Asian Indian
gender	female
growth mode	Suspension
karyotype	Not specified
morphology	Lymphoblastoid
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice

RIDADR	NONH for all modes of transport
WGK Germany	3

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THAI 742 human cell line

A: 2403 3303
Cw: 0102 03
B: 4601 1512

HLA A: 2403 33
HLA A ief:
HLA C: 1 3
HLA B: 46 76

RPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.

biological source	human blood
ethnicity	Thai
gender	not specified
growth mode	Suspension
karyotype	Not specified
morphology	Lymphoblastoid
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice

Mouse J558L + Mouse VH gene + Human C(gamma 3) gene

RIDADR	NONH for all modes of transport
WGK Germany	3

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THG3-B5-13

This human chimeric antibody is specifically directed against the hapten-4-hydroxy-3-nitrophenacetyl. V lambda of the endogenous light chain of plasmacytoma line complements VNP of transfected heavy chain to yield specific antibody. Gene marker: gpt.

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Mouse + Human Transfectoma
ImmunoDon	Not applicable
Myeloma	J558L
Reactivity	Anti-NP (4-hydroxy-3 nitrophenacetyl)
application(s)	cell culture \| mammalian: suitable
isotype	IgG3 G3m(g)
shipped in	dry ice
storage temp.	−196°C

Mouse J558L + Mouse VH gene + Human C(gamma 3) gene

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THG3-MP-2-19-3-8

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Mouse + Human Transfectoma
ImmunoDon	Not applicable
Myeloma	J558L
Reactivity	Anti-NP (4-hydroxy-3 nitrophenacetyl)
application(s)	cell culture \| mammalian: suitable
isotype	IgG3 G3m(b)
shipped in	dry ice
storage temp.	−196°C

Human monocytic leukaemia

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THO, MA human cell line

Кат. номер

94082258-RNA-5UG

94082258-DNA-5UG

94082258-CDNA-20UL

94082258-1VL

General description
Homozygous: No
Consanguineous: No
Cell Line Description
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
DNA Profile
A: 02 11
B: 07 27
HLA DRB1: 0401 1501
HLA DRB4: 101
HLA DRB5: 101
HLA DQAP: 1.2 3.1
HLA DQA1: 0102 03
HLA DQB1: 0301 0602
HLA DPB1: 401
Serological Profile
HLA A: 2 11
HLA A ief: 2.2 11.2
HLA C: 2 7
HLA B: 7 27
HLA B ief: 7.2 27.4
Bw4/bw6: 4 6
Culture Medium
RPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
Subculture Routine
Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.
Параметры

biological source human blood

ethnicity Caucasian

gender male

growth mode Suspension

karyotype Not specified

morphology Lymphoblastoid

application(s) cell culture | mammalian: suitable

shipped in dry ice

Safety Information

RIDADR NONH for all modes of transport

WGK Germany 3

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THP 1 Cell Line human

Derived from the peripheral blood of a 1 year old male with acute monocytic leukaemia. THP-1 cells have Fc and C3b receptors and lack surface and cytoplasmic immunoglobulins. These cells also stain positive for -napthhyl butyrate esterase, produce lysozymes and are phagocytic (both latex beads and sensitised erythrocytes). THP-1 cells can also restore the response of purified T lymphocytes to Concanavlin A, show increased CO2 production on phagocytosis and can be differentiated into macrophage-like cells using for example DMSO.Growing orders are recommended due to difficulties that can be experienced during the initial start-up of this cell line. Please note: We routinely resuscitate and culture this cell line with success, however, some laboratories have found this cell line difficult to establish following resuscitation from a frozen ampoule. Our Quality Control (QC) Department strongly recommend that this line is supplied as a Growing culture whenever possible e.g. to locations within or near the European Union. We routinely resuscitate and maintain this cell line growing in our laboratory to enable us to supply growing cultures quickly. If however, you still wish to receive the cell line Frozen we can supply but our normal replacement Policy will not apply should you encounter resuscitation difficulties.We recommend, if you are in the European Union (where we can deliver growing cultures), you select a Growing culture.

THP 1 cell line human has been used to study:

bactericidal activity of moxifloxacin against Staphylococcus aureus
the effect of lipopolysaccharide-mediated inflammatory response in murine versus human cell lines
the effects of purine nucleosides on the in vitro growth of Cryptosporidium parvum
the change in gene expression after infection with Mycobacterium tuberculosis
the induction of cytokines in the cell line in presence of antigens obtained from Clostridium difficile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 13
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 8,9.3
TPOX: 8,11
vWA: 16

RPMI 1640, + 2mM Glutamine, + 10% Foetal Bovine Serum (FBS / FCS),.

If starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube, e.g. 15ml tube, slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 100μl, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and resuspend the cell pellet at a density of 3 - 5 x 100,000 cells/ml in fresh medium containing 20% serum. Incubate flask at 37°C; 5 - 7% CO2. Check daily. Keep flask in a vertical position until the cells reach the exponential phase of growth. This can take up to 7 days. Once the culture is established the serum concentration can be reduced to 10%. To keep the cells in exponential growth, maintain cultures between 3-8x100,000 cells/ml. Requires 5% DMSO and 95% fetal bovine serum (FBS) as cryoprotectant. Growing orders are recommended due to difficulties that can be experienced during the initial start-up of this cell line. Replacements will be charged at full cost where claims cannot be substantiated.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	human blood
growth mode	Suspension
karyotype	Not specified
morphology	Monocyte
products	Not specified
receptors	Fc, C3b
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

RIDADR	NONH for all modes of transport
WGK Germany	3

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TISI human cell line

Кат. номер

88052042-RNA-5UG

88052042-CDNA-20UL

88052042-DNA-5UG

88052042-1VL

General description
Consanguineous: Yes
Cell Line Description
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
DNA Profile
A: 2402
Cw: 401
B: 3508
HLA DRA: 101
HLA DRB1: 1103
HLA DRB3: 202
HLA DQAP: 4.1
HLA DQA1: 501
HLA DQB1: 301
HLA DPA1: 1
HLA DPB1: 402
Serological Profile
HLA A: 24
HLA A ief: 24.1
HLA C: 4
HLA B: 35
HLA B ief: 35.2
Bw4/bw6: 6
HLA Dw: TISI
HLA DR: 11
HLA Dw2: 25
HLA DR52: 52
HLA DQ: 7
HLA DP: 4
Culture Medium
RPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
Subculture Routine
Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.
Параметры

biological source human blood

ethnicity French

gender female

growth mode Suspension

karyotype Not specified

morphology Lymphoblastoid

application(s) cell culture | mammalian: suitable

shipped in dry ice

Safety Information

RIDADR NONH for all modes of transport

WGK Germany 3

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TK-TS 13

Hamster Syrian kidney

This line is a thymidine kinase deficient mutant of a ts 13, a temperature-sensitive mutant of BHK 21. Reversion rate is very low but the cells can be reselected in BUdR.

Transfection studies

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	hamster kidney
growth mode	Adherent
karyotype	Not specified
morphology	Epithelial
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice
storage temp.	−196°C

Human lymphoblast, thymidine kinase heterozygote

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TK6

The thymidine kinase heterozygote cell line TK6 has been isolated from the lymphoblastoid line HH4. HH4 was derived from the WIL-2 cell line. TK6 cells are CD19 positive, 50% of the cell population express CD20 and a small sub-population has been tested positive for CD22. We cannot confirm the BVDV status of this cell line.

Bromodeoxyuridine resistant assays
TK-6 cell line has been used to study the in vitro genotoxic effects of trans- and cis-2-ethylhexyl 4-methoxycinnamate (EHMC). It has also been used to study the interaction of silver nanomaterials with the cells.

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 12,13
D7S820: 9,11
THO1: 8,9.3
TPOX: 8,11
vWA: 17,20

RPMI 1640 + 2mM Glutamine + 10% Horse Serum (HS) or FBS.

Maintain cultures between 1-9 x 100,000 cells/ml; 5% CO2; 37°C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	human lymph
growth mode	Suspension
karyotype	Not specified
morphology	Not specified
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice
storage temp.	−196°C

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TK6TGR

Derived from the human lymphoblast TK6, which is heterozygous for TK. The parent line was exposed to 20ug/ml thioguanine which produced a thioguanine resistant sub-population. The cells can be used as a fusion partner.

Fusion partner

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 12,13
D7S820: 9,11
THO1: 8,9.3
TPOX: 8,11
vWA: 17,20

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	human blood
growth mode	Suspension
karyotype	Not specified
morphology	Lymphoblast
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice
storage temp.	−196°C

Mouse cornea epithelial progenitor cells

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TKE2

TKE2 is the first cell line of mouse corneal epithelial progenitor cells isolated from the basal layer of the limbal epithelium.TKE2 has applications in the cell biology of corneal epithelial cells, toxicity, and differentiation. TKE2 can be induced to differentiate into stratified epithelium on increasing the calcium concentration and addition of foetal bovine serum.

Not specified

Stemline Keratinocyte Medium II (Sigma S0196) + Stemline Keratinocyte Growth Supplement (Sigma S9945) + 2 mM Glutamine.

Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 2 x 102 - 2 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor. To remove all traces of trypsin and trypsin inhibitor pellet the cells by centrifugation and resuspend the cells in fresh medium. Media change every second day.

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Mouse Sp2/0 x Mouse BALB/c spleen

biological source	eye, mouse (CD-1)
gender	female
growth mode	Adherent
karyotype	Normal karyotype of the female mouse CD1 strain
morphology	Epithelial; small and regular cobblestone-like shape
products	Stains positive for nuclear p63 expression and K14 keratin.
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice

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TL human cell line

Кат. номер

94050310-CDNA-20UL

94050310-1VL

94050310-RNA-5UG

94050310-DNA-5UG

General description
Homozygous: No
Consanguineous: No
Cell Line Description
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
DNA Profile
A: 0201 2402
Cw: 0401 0602
B: 3508 1302
HLA DRB1: 0701 0301
HLA DQA1: 0201 0501
HLA DQB1: 0201 0201
Serological Profile
HLA A: 2 24
HLA C: 4 6
HLA B: 35 13
HLA DR: 3 7
HLA DQ: 2 2
Culture Medium
RPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
Subculture Routine
Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.
Параметры

biological source human blood

ethnicity Caucasian

gender male

growth mode Suspension

karyotype Not specified

morphology Lymphoblastoid

application(s) cell culture | mammalian: suitable

shipped in dry ice

Safety Information

RIDADR NONH for all modes of transport

WGK Germany 3

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TM1

The antibody is cytotoxic for Leu-5 positive cells. The cells require NaP, oxaloacetic acid and bovine insulin in the growth medium.

Human lymphocytes (HLA phenotype A2, A28, B27, Bw4, Cw2)

RPMI 1640 + 2mM glutamine + 1% Non Essential Amino Acids (NEAA) + 0.05mM 2-mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	Mouse x Mouse Hybridoma
ImmunoDon	BALB/c mouse spleen
Myeloma	Sp2/0
Reactivity	Anti-Leu-5
application(s)	cell culture \| mammalian: suitable
isotype	IgM
shipped in	dry ice
storage temp.	−196°C

Mouse BALB/c testis, Leydig cell

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TM3

Originated from primary cultures of Leydig cell enriched preparations from normal testes of 11-13 day old BALB/c nu/+ mice. Characteristics similar to primary cultures of Leydig cells. Responds to leuteinising hormone but not FSH. In the presence of LH the cells are capable of metabolising cholesterol. These cells are non-tumourigenic.

Hormone studies

Ham′s F12 + DMEM (1:1) + 2mM Glutamine + 5% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:5 to 1:200 i.e. seeding at 1x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.

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biological source	mouse testis
growth mode	Adherent
karyotype	Not specified
morphology	Not specified
products	Prostaglandin F2a
receptors	Epidermal growth factor (EGF), Androgen, Oestrogen, Progesterone
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice
storage temp.	−196°C

Mouse BALB/c testis sertoli cell

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TM4

From primary culture of sertoli cell enriched preparations from normal testes of a 11-13 day old BALB/c nu/+ mice. Cells respond to FSH but not LH; Plasminogen activator secretion is stimulated by FSH and also retinoic acid. Note: only distributed frozen.

Hormone studies

Ham′s F12 + DMEM (1:1) + 2mM Glutamine + 5% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:20 to 1:200 i.e. seeding at 1x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Cells resuscitated from frozen stock should be split 1:3 at the first passage and thereafter as above.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	mouse testis
growth mode	Adherent
karyotype	Not specified
morphology	Not specified
products	Transferrin, H-Y Ag, novel retinol-binding protein
receptors	Androgen, oestrogen and progesterone receptors
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice
storage temp.	−196°C

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TO human cell line

A: 6802 3001
Cw: 0602 15
B: 1302 5101

HLA A: 68 30
HLA A ief: 68.2
HLA C: 6
HLA B: 13 51
Bw4/bw6: 4

RPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.

biological source	human blood
ethnicity	Not specified
gender	not specified
growth mode	Suspension
karyotype	Not specified
morphology	Lymphoblastoid
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice

Human ovarian cancer; oestrogen receptor negative, drug efficacy and toxicity

RIDADR	NONH for all modes of transport
WGK Germany	3

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TO14

TO14 is an adherent cell line derived from a metastasis to the omentum obtained at hysterectomy from a patient with a well differentiated serous adenocarcinoma. TO14 exhibits poor growth in semi-solid medium (agar). TO14 is from the same patient as PEO23 and PEO14 cell lines (Sigma catalog numbers 10032313 and 10032311 respectively).
This cell line is one of nine from the PE ovarian adenocarcinoma panel (derived from 4 patients at varying stages of ovarian cancer, isolated from various malignant sites, and at various treatment stages) available at ECACC which provides a model system for research into the mechanism of oestrogen action on ovarian adenocarcinoma tumour cells, and for the study of efficacy and toxicity of oestrogen protagonists.

Not specified

RPMI 1640 + 2 mM Glutamine + 2 mM Sodium Pyruvate + 10% Fetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2- 3 x 104 cells/cm2<sup/>

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

biological source	human ovary
growth mode	Adherent
karyotype	For modal chromosome numbers see Langdon SP et.al. (1988) Cancer Research Nov 1; 48(21):6166-72.
morphology	Epithelial; stellate in shape and producing hemicystic colonies which grow upwards from the substrate and detach as spheres.
products	Oestrogen receptor negative cell line.
receptors	Oestrogen receptor negative cell line. For antigen expression see Langdon SP et.al. (1988) Cancer Research Nov 1; 48(21):6166-72.
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	metastasis
shipped in	dry ice

RIDADR	NONH for all modes of transport
WGK Germany	3

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TOKUNAGA human cell line

A: 2402
HLA DRB1: 1502
HLA DRB5: 102
HLA DRB6: 2
HLA DQA1: 103
HLA DQB1: 601
HLA DPA1: 2
HLA DPB1: 901

HLA A: 24
HLA B: 52
Bw4/bw6: 4
HLA Dw: 12
HLA DR: 15
HLA DR51: 51
HLA DQ: 6

RPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.

biological source	human blood
ethnicity	Not specified
gender	male
growth mode	Suspension
karyotype	Not specified
morphology	Lymphoblastoid
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice

Human B lymphocyte, EBV transformed

RIDADR	NONH for all modes of transport
WGK Germany	3

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TOU (TOU I-2)

Peripheral blood lymphocytes from a male Tunisian immortalised by EBV transformation. The cell line contains the following deletions, in the human immunoglobulin heavy chain constant region locus (IGH chromosome 14q32.3): deletion II (del EP1-A1-GP) Gm-Am genotype Gm b; -; -A2m2/Gm g; za; .A2m2. Gm allotypes are indicated in the order G3m; G1m; G2m. The absence of G1m and G2m allotypes due to the deletion of the IGHG1 and IGHG2 genes is indicated by (-). Only one allotype is defined on the gamma 2 chain and may or may not be present; (.) indicates the absence of this allotype.

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Maintain cultures at 5-9x100,000 cells/ml; 5% CO2; 37°C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	human blood
growth mode	Suspension
karyotype	Not specified
morphology	Lymphoblast
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

Fish Rainbow Trout Pronephric Stroma

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TPS

TPS has been initiated from a long-term pronephric stroma culture of an adult 1 year old rainbow trout (Oncorhynchus mykiss W). The cells grow at 4° to 22°C, with an optimum temperature of 18°C. They do not form true monolayers, but overlap in a multilayered network of fibroblastic, epithelioid and giant cells. TPS cells are susceptible to viral haemorrhagic septicemia virus (VHSV) and infectious haemopoietic necrosis virus (IHNV). They have been applied to support the development of haemopoiesis when co-cultured with haemopoietic precursors. Spontaneous angiogenesis processes in vitro occurred in TPS cultures when they are kept in the same flask, i.e. without passages, for long times (over 2 months).

Fish Haemopoiesis and Fish pathology.

RPMI 1640 + 2mM Glutamine + 25mM Hepes + 2.5mM NaP + 0.01mM Hydrocortisone-21-hemisuccinate + 0.088mM Guanosine + 0.0225mM Cytidine + 0.094mM Adenosine + 0.102mM Uridine + 10% FBS + 10% HS, pH 7.4-7.6. At ECACC successful growth of this cell line has bee

Split sub-confluent cultures (70-80%) 1:2 to 1:4 using 0.25% trypsin or trypsin/EDTA; trypsinise at room temperature once a week; air atmosphere; 18°C. Fish cell lines detach easily during transit if the culture is very young. After resuscitation split cells 1:3 to 1:4 and culture for at least one week with 1-2 media changes before shipping.

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biological source	fish kidney (anterior)
growth mode	Adherent
karyotype	Modal no. 58
morphology	Fibroblast-like/Epithelial-like
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice
storage temp.	−196°C

The TR146 cells are appropriate for drug transport studies and they mimic normal human buccal epithelium. It serves as an oral mucosa model for barrier property studies with various molecules including tight junction proteins, aquaporins and epithelial-mesenchymal transition (EMT) markers.

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TR146 cell line human 10032305-1VL

General description

Cell Line Origin

Human squamous cell carcinoma

TR146 was derived from the neck node of a 67 year-old female (primary tumour was sited in buccal mucosa). The patient had had previous radiotherapy (6,000 rads) and neck dissection. TR146 is tumourigenic in female (nu/nu) mice leading to the production of rapidly progressing tumours. Histology: well-differentiated keratinizing squamous cell carcinoma.

TR146 cell line human has been used:

in cytotoxicity studies with an antibacterial compound, metronidazole
in viability and metabolic studies post blue laser light exposure,
as an oral epithelium-resembling cell line model for in vitro C. albicans infection studies
in barrier function studies using transepithelial electrical resistance (TEER)
for the transport studies with C-reactive protein (CRP)

STR-PCR Data: Amelogenin: X
CSF1PO: 11,13
D13S317: 11,14
D16S539: 13
D5S818: 10,11
D7S820: 10,12
THO1: 9
TPOX: 8,9
vWA: 15,18

HAMS F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density of 5 x 104 cells/cm2.

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Quality Level	100,
biological source	human mouth
description	Human squamous cell carcinoma
growth mode	Adherent
karyotype	Not specified
morphology	Polygonal
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	196°C

Rat Wistar-Furth oligodendroglioma

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable

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TR33B

Ethyl nitrosourea induced tumour of the spinal cord and roots from a Wistar-Furth rat. In cell culture the cells grow to a high density and form a sheet of cells with many processes. TR33B is the parent line of 33B (Sigma Catalogue number. 85081901).

DMEM + 2mM Glutamine + 10% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

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biological source	rat brain
growth mode	Adherent
karyotype	Not specified
morphology	Glial
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

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TR6Bc1

Mouse C3H.He Schwannoma

Ethyl nitrosourea induced tumour in C3H. The mice, originated in trigeminal nerve and brain; readily transplanted and resembles schwannoma histologically. Cultured cells form prominent processes, resemble 33B (Sigma Catalogue number. 85081901) morphologically, contain abundant C type particles.

DMEM + 2mM Glutamine + 10% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	mouse nerve
growth mode	Adherent
karyotype	Not specified
morphology	Glial
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

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TRA-171

Insect larvae, mosquito

The cell line TRA-171 has been established from freshly hatched larvae of Toxorhynchites amboinensis. After eggs had been surface-sterilised, they were allowed to hatch in MM / VP12 medium and were mechanically damaged at the anterior or posterior ends of the body in 0.25% trypsin. After washing and cultivation at 22 - 25°C small patches of cells were visible leading to the isolation of the cell line TRA-171 after 72 days in culture. The cells have been adapted to grow in a 1:1 mixture of Leibovitz L-15 and Mitsuhashi and Maramorosch media plus supplements. Four serotypes of laboratory adapted and three type strains (1, 2 and 3) of un-adapted dengue virus will replicate in the cells at 28°C.

Virus studies: Dengue virus

L15: Mitsuhashi and Maramorosch Insect Medium (1:1) + 2mM Glutamine + 0.05% BSA + 1% NEAA + 10% Heat Inactivated Foetal Bovine Serum (HI-FBS)

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4 x 10,000 cells/cm2 using 0.25% trypsin for 5 minutes only at 28°C, after quenching the trypsin with fresh medium dislodge cells by gentle scraping; CO2 not required; 28°C. Fr

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biological source	insect larvae
growth mode	Adherent
karyotype	Not specified
morphology	Fibroblast-like
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice
storage temp.	−196°C

Human embryonal kidney, SV40 transformed

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Trex1 KO Cell line from mouse

Кат. номер

12021509-1VL

12021509-RNA-5UG

12021509-DNA-5UG

Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
Параметры

biological source mouse embryo

growth mode adherent

morphology fibroblast

shipped in dry ice

storage temp. −196°C

Safety Information

RIDADR NONH for all modes of transport

WGK Germany 3

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tsa201 Cell Line human

tsA201 is a transformed human kidney (293, Sigma catalogue no. 85120602) cell line stably expressing an SV40 temperature-sensitive T antigen. The cell line has been used in a variety of functional expression assays and has been reported to produce high levels of recombinant proteins. Transfection efficiency decreases over a period of several months, therefore it is advisable to lay down a frozen stock as a reserve. The cells have been eradicated from mycoplasma at ECACC. The identity of tsA201 and 293 has been confirmed by STR profiling.

tsa201 human cell line has been used for protein-binding studies, whole-cell patch clamp recordings and transfection studies.

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 9,13
D5S818: 8,9
D7S820: 11
THO1: 7,9.3
TPOX: 11
vWA: 16,19

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:8; cells are dislodged by mechanical agitation or EDTA solution; 5% CO2; 37°C. Do not let cells become over confluent as this can affect morphology.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

biological source	human kidney
growth mode	Adherent
karyotype	Not specified
morphology	Epithelial-like
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice
storage temp.	−196°C

Immortalised mouse dendritic cell line

RIDADR	NONH for all modes of transport
WGK Germany	3

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tsDC

Not specified

IDMEM (I3390), + 2 mM L-Glutamine (G7513), + 5% FBS (F2442), + 100U/ml Penicillin + 100 ug/ml Streptomycin + 0.05 mM 2ME (M6250),

Passage cells approximately once every 2 weeks. Culture at 33 °C; 5-10% CO2. Cells are of heterogenous morphology, but the line has been cloned. Some cells adhere and others grow in suspesion. Cells are detached by short incubation in PBS plus 20mM EDTA, followed by washing in culture medium. Despite the cells slow growth, they can be highly diluted -growth does not depend on a minmum cell density.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Legal Information

This material is cited in US and/or other patent and may not be used to infringe patent claims. US patent No′s. 5688692, 5866759 and international patent pending PCT/GB91/00262.

This cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.

biological source	mouse bone
growth mode	Semi-adherent
karyotype	Not specified
morphology	Not specified
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
shipped in	dry ice

Human Caucasian thyroid medullary carcinoma

RIDADR	NONH for all modes of transport
WGK Germany	3

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Established from a thyroid medullary carcinoma of a 77 year old Caucasian female. Tumourigenic in nude mice.

STR-PCR Data: Amelogenin: X
CSF1PO: 10,13
D13S317: 11
D16S539: 12,13
D5S818: 12,13
D7S820: 10,12
THO1: 6,9
TPOX: 8,11
vWA: 16,18

Ham′s F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Population doubling is very slow, cells can take up to 5 days to reach 50% confluency; media change 2-3 times a week is recommen

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biological source	human thyroid
growth mode	Adherent
karyotype	Aneuploid karyotype with marker chromosomes
morphology	Not specified
products	Calcitonin, Carcinoembryonic antigen (CEA)
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

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TUBO human cell line

Кат. номер

88052045-DNA-5UG

88052045-RNA-5UG

88052045-CDNA-20UL

88052045-1VL

General description
Consanguineous: No
Cell Line Description
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
DNA Profile
A: 0216 0301
Cw: 0704 1502
B: 5101
HLA DRA: 0101 0102
HLA DRB1: 1104 1201
HLA DRB3: 202
HLA DQA1: 501
HLA DQB1: 301
HLA DPA1: 1
HLA DPB1: 02012 0402
Serological Profile
HLA A: 2 3
HLA A ief: 2.3 3.2
HLA C: 7
HLA B: 51
HLA B ief: 51
Bw4/bw6: 4
HLA Dw: 5 DB6
HLA DR: 11 12
HLA Dw2: 25
HLA DR52: 52
HLA DQ: 7
HLA DP: 2 4
Culture Medium
RPMI 1640, (R0883) + 2mM Glutamine, (G7513) + 20% Foetal Bovine Serum (FBS,) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
Subculture Routine
Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.
Параметры

biological source human blood

ethnicity French

gender female

growth mode Suspension

karyotype Not specified

morphology Lymphoblastoid

application(s) cell culture | mammalian: suitable

shipped in dry ice

Safety Information

RIDADR NONH for all modes of transport

WGK Germany 3

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U-2 OS Cell Line human

Cell line derived in 1964 from a moderately differentiated sarcoma of the tibia of a 15 year old girl.

U-2 OS has been used to study:

the importance of cyclin D1 for the activity of lithocholic acid hydroxyamide (LCAHA)
the interaction of human single-stranded DNA binding protein 1 (hSSB1) with bloom syndrome protein helicase (BLM helicase)
calcium-mediated actin reset (CaAR) in response to physiological changes

STR-PCR Data: Amelogenin: X
CSF1PO: 13
D13S317: 13
D16S539: 11,12
D5S818: 11
D7S820: 11,12
THO1: 6,9.3
TPOX: 11,12
vWA: 14,18

McCoy′s 5a medium with 1.5 mM Glutamine; 10% Foetal Bovine Serum (FBS).

Split subconfluent 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new culture flasks.

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biological source	human bone
growth mode	Adherent
karyotype	Not specified
morphology	Not specified
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

Human glioblastoma astrocytoma

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U-251 MG cell line human

Derived from a malignant glioblastoma tumour by explant technique. U-251 was formerly distributed as U-373 MG (Sigma product no. 89081403) until short tandem repeat (STR)-PCR profiling confirmed identity with U-251. A new deposit of U-373 MG known as U-373 MG (Uppsala) is now available (Sigma product no. 08061901).Background to the identity query for the cell line U-373 MG: The American Type Culture Collection (ATCC) reported that their stock of U-373 MG had been shown to have differing genetic properties to stock from the originator′s laboratory, and to share similarities with another glioblastoma cell line, U-251. In light of this, ECACC undertook an investigation into the authenticity of its own stock of the U-373 MG cell line. ECACC found similar results to the ATCC i.e. the stock held as U-373 MG was found to be identical by STR-PCR profiling to U-251. The U-373 MG cell line listed under product no. 89081403 has been re-named as ′U-251 (formerly known as U-373 MG)′ and has the new Sigma product no. 09063001.

U-251 MG cell line human has been used:

for the extraction of cancer stem cells from U-251 human glioblastoma cell line
to study the mechanism of Pax6 (paired box protein)-associated increase in expression of Dkk3 (Dickkopf 3)
to study the role of JARID1B (jumonji AT-rich interactive domain 1B) in the pathogenesis of glioma
to study the therapeutic efficacy of Irinophore C combined with temozolomide in glioblastoma tumor model

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 10,11
D16S539: 12
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 16,18

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

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biological source	human brain
growth mode	Adherent
karyotype	2n = 46
morphology	Pleomorphic/astrocytoid
products	Contains GFAP positive cells
receptors	PDGFR alpha and EGFR. See Nistr M. et al., (1988) & (1991)
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

Human glioblastoma astrocytoma

RIDADR	NONH for all modes of transport
WGK Germany	3

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U-87 MG Cell Line human

Derived from a malignant glioma from a female patient by explant technique. It is reported to produce a malignant tumour consistent with glioblastoma in nude mice.

U-87 MG cell line has been used to study the effects of the lysosomal destabilizing drug Siramesine on glioblastoma. It has also been used to study calcium-independent disruption of microtubule dynamics by nanosecond pulsed electric fields.
Neural studies

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 8,11
D16S539: 12
D5S818: 11,12
D7S820: 8,9
THO1: 9.3
TPOX: 8
vWA: 15,17

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

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biological source	human brain (glioblastoma astrocytoma)
growth mode	Adherent
karyotype	2n = 46
morphology	Epithelial-like
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

Human bladder transitional cell carcinoma

RIDADR	NONH for all modes of transport
WGK Germany	3

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U-BLC1

Spontaneously immortal cell line established from a primary transitional cell carcinoma of the bladder. Derived from a female patient.

2/3 DMEM:1/3 RPMI + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. The medium must be changed to fresh medium every 48 hours.

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biological source	human bladder
growth mode	Not specified
karyotype	Aneuploid, with specific marker chromosomes
morphology	Epithelial
products	Not specified
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

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U266B1

This cell line was derived from peripheral blood of a patient with an IgE myeloma (epsilon2,lambda2). The line has been reported to secrete IgE and is used as fusion partner for the production of hybridomas. U266B1 has been reported to produce interleukin-6 (IL-6).

Fusion partner

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12,13
D13S317: 12
D16S539: 10
D5S818: 11,12
D7S820: 11,12
THO1: 5,7
TPOX: 8
vWA: 17

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

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biological source	human blood
growth mode	Suspension
karyotype	Not specified
morphology	Lymphoblast
products	IgE
receptors	Not specified
application(s)	cell culture \| mammalian: suitable
relevant disease(s)	cancer
shipped in	dry ice
storage temp.	−196°C

Mouse Sp2/0 x Mouse BALB/c spleen

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U28

The hybridoma U28 was obtained by fusing SP2/0 myeloma cells with spleen cells from BALB/c mice that had been given repeated intranasal infection with a single i.v. injection before fusion. The antibody binds murine IgG2a of "a" allotype and the Fc region. There is no cross reactivity with A/PR/8/34 influenza virus, DNP, NP or DNA cytoskeletal proteins. Contains a CH2 domain.

A/PR/8/34 mouse pathogenic human influenza virus

Differential detection of complexed IgG2a in the presence of native IgG2a, allotype detection or quantification

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C

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biological source	Mouse x Mouse Hybridoma
ImmunoDon	BALB/c mouse spleen
Myeloma	Sp2/0
Reactivity	Anti-IgG2a, mouse
application(s)	cell culture \| mammalian: suitable
isotype	IgM
shipped in	dry ice
storage temp.	−196°C