Phosphatase, Alkaline from Escherichia coli

CAS Number:	9001-78-9
Enzyme Commission (EC) Number:	3.1.3.1 ( BRENDA | IUBMB )
EC Number:	232-631-4
MDL number:	MFCD00131849
eCl@ss:	42010105
NACRES:	NA.54

Кат. номер

P5931-200UN-PW

P5931-500UN-PW

P5931-100UN-PW

P5931-200UN

P5931-100UN

P5931-500UN

P5931-1KU

P5931-50UN

Alkaline phosphatase (ALP) of E.coli is a member of an enzyme group that possesses intragenic complementation.
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P5931 has been used during immunoblots to treat cell membranes prior to Tau1 incubation.
The enzyme from Sigma has been used to develop a synthetic nanopore membrane. This membrane mimics protein channels that are regulated by phosphorylation/dephosphorylation and uses an aligned array of carbon nanotubes (CNTs) impregnated in a polystyrene matrix.
Packaging
100, 200, 500 units in glass bottle
Biochem/physiol Actions
Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.
The enzyme is a phosphohydrolase having an optimal pH of 10 in vitro. The actual optimum pH varies depending on the nature and concentration of the substrate, the type of buffer, the phosphate acceptor, and to some extent the nature of the isoenzymes. It catalyzes the hydrolysis of phosphate monoesters such as p-nitrophenyl phosphate, phenyl phosphate, phenolphthalein phosphate, -glycerol phosphate, -glycerol phosphate, 2-phosphorylglycerate, triosephosphate, glucose-6-phosphate, glucose 1-phosphate, fructose 1-phosphate, fructose 6-phosphate, adenosine 5-phosphate adenosine 3-phosphate, phosphoenolpyruvate, and -nicotinamide adenine dinucleotide phosphate. The activity is inhibited by 1,10-phenanthroline monohydrate, diethylenetriaminepentaacetic acid, ethylene glycol-bis(2-aminoethylether)-N,N,N,N-tetraacetic acid, and ethylenediaminetetraacetic acid disodium salt dihydrate.
Unit Definition
One unit will hydrolyze 1.0 µmole of p-nitrophenyl phosphate per min at pH 10.4 at 37 °C.
Physical form
Lyophilized powder containing Tris buffer salts, MgSO4, and ZnSO4
Preparation Note
Chromatographically purified

form	lyophilized powder
Quality Level	200,
specific activity	30-60 units/mg protein (in glycine buffer)
composition	Protein, ~50% biuret
storage temp.	20°C

Personal Protective Equipment	Eyeshields,, Gloves,, type N95 (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point F	Not applicable
Flash Point C	Not applicable