Anti-CBX2 Antibody, clone 3I14 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1084-25UL

ZRB1084-4X25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 3I14 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Chromobox protein homolog 2 (CBX2). It targets an epitope within 25 amino acids from the N-terminal region.
KLH-conjugated linear peptide corresponding to 25 amino acids from the N-terminal region of human Chromobox protein homolog 2 (CBX2).
Quality Control Testing
Evaluated by Western Blotting in HEK293 cell nuclear extract.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CBX2 in HEK293 cell nuclear extract.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CBX2 in nuclear extract from NIH3T3 cells, HeLa cells, and L6 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CBX2 in HeLa, A431, HepG2, and NIH 3T3 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CBX2 in human skin tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound CBX2 peptide with a KD of 8.7 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-CBX2, clone 3I14 ZooMAb®, Cat. No. ZRB1084, is a recombinant Rabbit monoclonal antibody that specifically targets CBX2 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Target description
Chromobox protein homolog 2 (UniProt: Q14781; also known as CBX2) is encoded by the CBX gene (Gene ID: 84733) in human. CBX2 is a component of a Polycomb group (PcG) multiprotein PRC1-like complex that is expressed during interphase and metaphase. It plays a role in the lineage differentiation of the germ layers in embryonic development and is involved in sexual development, acting as activator of NR5A1 expression. It is shown to be localized in distinct foci on chromatin and in chromocenters. CBX2 is reported to be expressed from week 9 to week 25 post-fertilization in both male and female gonads. It is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. Disruption of this gene in murine models is shown to result in male-to-female gonadal sex reversal. Levels of CBX2 are reported to decrease in macrophages upon viral infection and knockdown of CBX2 gene in macrophages can inhibit virus-induced production of interferon-. CBX2 is reported to bind and recruit Lysine demethylase 6B (Jmjd3) to the IFN- promoter, leading to demethylation of H3K27me3 and increased transcription of IFN-. Mutations in CBX2 gene are associated with gonadal dysgenesis in humans. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Sun, D., et al. (2019). Protein Cell 10(4); 285-294).
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200,
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	3I14, monoclonal, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt ~ 56.08 kDa, observed mol wt ~ 56 kDa
species reactivity	mouse, human, rat
species reactivity (predicted by homology)	canine
packaging	antibody small pack of 25 µL
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	affinity binding assay: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
shipped in	ambient
storage temp.	2-8°C

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