Anti-Derlin-1 Antibody, clone 2C2, ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1356-4X25UL

ZRB1356-25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.,
Specificity
Clone 2C2 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Derling-1 protein. It targets an epitope within 14 amino acids from the C-terminal region.
KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal region of human Derlin-1.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Derlin-1 in NIH/3T3, FBHE, and L6 cell lysates.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Derlin-1 in HeLa, A431, HUVEC and NIH 3T3 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Derlin-1 in human small intestine tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Anti-Derlin-1, clone 2C2 ZooMAb, Cat. No. ZRB1356, is a recombinant Rabbit monoclonal antibody that specifically targets Derlin-1 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Target description
Derlin-1 (UniProt: Q9BUN8; also known as Degradation in endoplasmic reticulum protein 1, DERtrin-1, Der1-like protein 1) is encoded by the DERL1 (also known as DER1, UNQ243/PRO276) gene (Gene ID: 79139) in human. Derlin-1 is a multi-pass membrane protein that serves as an E3 ligase mediator that links target proteins to ubiquitin-mediated proteasomal degradation in the cytosol. It is a functional sub-unit of the ER-associated degradation (ERAD) that forms a retro-translocation channel in the ER membrane and functions in the recognition of misfolded membrane or transmembrane proteins and their subsequent degradation in the ubiquitin-proteasomal system. Before any ERAD proteins are processed by the cytoplasmic ubiquitin-proteasome system, they are translocated across the ER membrane through an AAA ATPase p97/VCP-driven process. Derlin-1 is also involved in endoplasmic reticulum stress-induced pre-emptive quality control, a mechanism that selectively attenuates the translocation of newly synthesized proteins into the endoplasmic reticulum and reroutes them to the cytosol for proteasomal degradation. Derlin-1 can form homo- and heterooligomers with Derlin-2 and -3, however, binding to Derlin-3 is shown to be DERL3 is lower. Derlin-1 contains three cytoplasmic domains (aa 2-22, 81-105, and 176-251), four transmembrane domains (aa 23-43, 60-80, 106-126, 155-175), and two lumenal domains (aa 44-59 and 127-154). This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: You, H., et al. (2017). J. Cell Sci. 130(6); 1027-1036).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	2C2, monoclonal, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt 28.8 kDa, observed mol wt ~18 kDa
species reactivity	human, bovine, rat, mouse
packaging	pkg of 25 µL
greener alternative product characteristics	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry,.
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
greener alternative category	Aligned,
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable