Anti-DHX37 Antibody, clone 3I16 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1524-25UL

ZRB1524-4X25UL

ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 3I16 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects DEAH box protein 37 (DHX37). It targets an epitope within 24 amino acids from the N-terminal region.
KLH-conjugated linear peptide corresponding to 24 amino acids from the N-terminal region of human DEAH box protein 37 (DHX37).
Anti-DHX37, clone 3I16 ZooMAb®, Cat. No. ZRB1524, is a recombinant Rabbit monoclonal antibody that targets DHX37 and is tested for use in is used in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DHX37 in HeLa cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected DHX37 in U2OS and Jurkat cell lysates.
Affinity Binding Assay: A representative lot of this antibody bound DHX37 with a KD of 2.3 x 10-10 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected DHX37 in human placenta and human skin tissue sections.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected DHX37 in MCF-7 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Probable ATP-dependent RNA helicase DHX37 (UniProt: Q8IY37; also known as EC:3.6.4.13, DEAH box protein 37) is encoded by the DHX37 (also known as DDX37, KIAA1517) gene (Gene ID: 57647) in human. DHX37, a member of the DEAD box helicase family, is an ATP-binding RNA helicase that plays a role in maturation of the small ribosomal subunit in ribosome biogenesis. It is required for the release of the U3 snoRNP from pre-ribosomal particles. Lack of DHX37 is reported to trigger a surveillance pathway that leads to degradation of pre-ribosomal particles and a depletion of DHX37 is shown to inhibit the conversion of the 21S pre-rRNA to 18SE. Its expression has been reported in fallopian tube, ovary, uterus, and testis and is also detected in the brain. Its DEAH box motif is localized to amino acids 372-375. It plays a role in early testis development and its pathogenic variants are reported to cause 46,XY gonadal dysgenesis and lead to testicular regression syndrome. Mutations in DHX37 gene are also reported to cause neurodevelopmental disorders with brain anomalies that are characterized by severe developmental delays, cortical volume loss and cerebellar hypoplasia. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: McElreavey, K., et al. (2020). Genet. Med. 22(1); 150-159; Choudhury, P., et al. (2019). RNA Biol. 16(1); 54-68).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	rabbit
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	3I16, recombinant monoclonal
description	recombinant, expressed in HEK 293 cells
product line	ZooMAb®
form	lyophilized
mol wt	calculated mol wt 129.55 kDa","observed mol wt ~150 kDa
purified by	using Protein A
species reactivity	human
species reactivity (predicted by homology)	monkey
packaging	antibody small pack of 25 µL
enhanced validation	recombinant expression
application(s)	affinity binding assay: suitable","immunocytochemistry: suitable","immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable","western blot: suitable
isotype	IgG
epitope sequence	N-terminal
conjugate	unconjugated
Protein ID accession no.	NP_116045,
UniProt accession no.	Q8IY37,
shipped in	ambient
storage temp.	2-8°C