Anti-DLC1 (C-terminal) antibody produced in rabbit SAB4200352-200UL

NACRES:

NA.41

Deleted in liver cancer 1 (DLC1) is a candidate tumor suppressor gene, originally obtained from human hepatocellular carcinoma (HCC). It is mapped to the human chromosome location 8p21-22. The DLC1 gene is majorly expressed in normal human tissues. It contains three major functional domains, sterile α-motif (SAM), Rho guanosine triphosphatase-activating protein (RhoGAP), and steroidogenic acute regulatory-related lipid transfer (START) domains. The N-terminal region of DLC1 localizes to focal adhesions by binding to Src Homology 2 (SH2) domains on tensins.
Specificity
Anti-DLC1(C-terminal) specifically recognizes human DLC1.
synthetic peptide corresponding to a sequence at the C-terminal of human DLC1, conjugated to KLH. The antibody is affinity-purified using the immunizing peptide immobilized on agarose.
Anti-DLC1 (C-terminal) antibody produced in rabbit may be used in immunoblotting.
Biochem/physiol Actions
Deleted in liver cancer 1 (DLC1) interacts with tensin2, a focal adhesion protein, localized at the end of stress fibers, which plays key roles in cytoskeletal organization and cell signaling. Mutations in the focal adhesion targeting (FAT) region of DLC1 reduces its expression and function. The focal adhesion localization of DLC1 is critical for cell motility and morphology. It is also critical for the tumor suppressor activity of DLC1 and under expressed in human hepatocellular carcinoma (HCC) and a variety of cancer types.
Physical form
Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	antigen ~200 kDa
species reactivity	human
enhanced validation	recombinant expression
concentration	~1.5 mg/mL
application(s)	western blot: 1-2 µg/mL using lysates of HEK-293T cells overexpressing human DLC1.
conjugate	unconjugated
UniProt accession no.	Q96QB1,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... DLC1(10395)

RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable