Anti-DLC1 (C-terminal region) antibody produced in rabbit SAB4200490-200UL

NACRES:

NA.41

Deleted in liver cancer 1 (DLC1), also known as ARHGAP7, is a candidate tumor suppressor gene, originally isolated from human hepatocellular carcinoma (HCC). DLC1 is mapped to chromosome 8p21.3-22 and is also expressed in normal human tissues. DLC1 protein comprises three domains namely steroidogenic acute regulatory related lipid transfer (START) domain, sterile a-motif (SAM) and Rho GTPase-activating protein (RhoGAP) domain.
Specificity
Anti-DLC1(C-terminal region) specifically recognizes human DLC1.
synthetic peptide corresponding to a sequence near the C-terminal of human DLC1 isoform 1, conjugated to KLH.
Anti-DLC1 (C-terminal region) antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence
• immunohistochemistry

Biochem/physiol Actions
Deleted in liver cancer 1 (DLC1) interacts with focal adhesion protein, tensin 2. DLC1 gene locus is recurrently deleted in solid tumors as well as in hepatocellular carcinoma (HCC). The Rho GTPase-activating protein (RhoGAP) region is responsible for the growth inhibitory effect in HCC. It has been shown that the N-terminal region of DLC1 localizes to focal adhesions by binding to SH2 domains on tensins. Mutations in the focal adhesion targeting (FAT) region of DLC1 reduces its expression and function. The cell motility and morphology maintenance functionality of DLC1 is primarily mediated by steroidogenic acute regulatory protein (STAR)- GTPase-activating proteins (GAP1) region.
Physical form
Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	antigen ~200 kDa
species reactivity	human
enhanced validation	recombinant expression
concentration	~1.0 mg/mL
application(s)	immunohistochemistry: 10-20 µg/mL using formalin-fixed paraffin embedded human colon.","immunoprecipitation (IP): 5-10 µg using lysates of HEK-293T cells overexpressing human DLC1.","indirect immunofluorescence: 1-2 µg/mL using MDBK cells.","western blot: 1.5-3 µg/mL using lysates of HEK-293T cells overexpressing human DLC1.
conjugate	unconjugated
UniProt accession no.	Q96QB1,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... DLC1(10395)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable