Anti-Doublecortin Antibody, clone 3F11 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1863-25UL

ZRB1863-4X25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 3F11 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Doublecortin. It targets an epitope within 17 amino acids from the C-terminal region.
KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human Doublecortin.
Quality Control Testing
Evaluated by Western Blotting in SH-SY5Y cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Doublecortin in SH-SY5Y cell lysate.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Doublecortin in mouse brain tissue sections.
Immunocytochemistry Analysis: A 1:10 dilution from a representative lot detected Doublecortin in Neuro-2a and U-87 MG cells.
Immunofluorescence Analysis: A 1:10 dilution from a representative lot detected Doublecortin in frozen mouse brain tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Doublecortin, clone 3F11 ZooMAb®, Cat. No. ZRB1863, is a recombinant Rabbit monoclonal antibody that detects Doublecortin and tested for use in Western Blotting, Immunofluorescence, Immunocytochemistry, and Immunohistochemistry (Paraffin).
Target description
Neuronal migration protein doublecortin (UniProt: O43602; also known as Doublin,Lissencephalin-X, Lis-X) is encoded by the DCX (also known as DBCN, LISX) gene (Gene ID: 1641) in human. Doublecortin is a microtubule-associated protein that is required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. It is expressed almost exclusively in immature neurons. Hence, it has emerged as an important marker of neurogenesis. It is highly expressed in newly produced cells in the neurogenic zones; the sub ventricular zone along the lateral ventricle and the subgranular zone (SGZ) of the dentate gyrus. Doublecortin binds to microtubules in migrating cells and as a result promote the movement of these cells. It is reported to direct neuronal migration by regulating the organization and stability of microtubules. Doublecortin may act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein and may participate in a signaling pathway that is crucial for neuronal interaction before and during migration. It undergoes phosphorylation by MARK1, MARK2, and PKA and the phosphorylation regulates its ability to bind microtubules. Doublecortin is reported to be involved in cancer metastasis where unstable interactions between doublecortin and microtubules destabilize cytoskeletal organization leading to disorganized movements of cells that can lead to the uncontrolled migration of cancer cells. Mutations in DCX gene are reported to cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, cognitive disability, subcortical band heterotopia. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200,
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	3F11, monoclonal, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt ~ 40.57 kDa, observed mol wt ~ 42 kDa
species reactivity	human, mouse
species reactivity (predicted by homology)	rat
packaging	antibody small pack of 25 µL
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable