Anti-Doublecortin (N-terminal) antibody produced in rabbit

NACRES:

NA.41

Кат. номер

D9943-200UL

D9943-25UL

Doublecortin gene is mapped to human chromosome Xq23. It comprises two homologous doublecortin (DC) domains, one each in the N-terminal and C-terminal region. A linker connects a flexible unstructured region. The C-terminal sequences also have serine/proline-rich region.
Specificity
Anti-Doublecortin(N-terminal) specifically recognizes human, rat, and mouse Doublecortin.
synthetic peptide corresponding to amino acids 1-17 located at the N-terminus of rat doublecortin, conjugated to KLH. This sequence is identical in mouse, and highly conserved in human doublecortin.
Anti-Doublecortin antibody produced in rabbit is suitable for immunoblotting at a working concentration of 0.5-1μg/mL using HEK-293T cells expressing human doublecortin and rat brain extract (S1 fraction), and 1-2μg/mL using mouse brain extract (S1 fraction).
Biochem/physiol Actions
Doublecortin is a 40kDa microtubule-associated protein (MAP) that is essential for migration of neurons during the development of the cerebral cortex. Mutations in the human DCX gene cause lissencephaly (smooth brain) or subcortical laminar heterotopia (SCLH), characterized by mental retardation and seizures. DCX interaction with MAPs stabilizes microtubules (MTs). The interaction with MTs is via a conserved N-terminal doublecortin (DC) domain. DCX can be regulated by phosphorylation at multiple sites by several kinases, including JNK, Cdk5, PKA, and MARK/PAR-1 family of protein kinases.
Physical form
Solution in 0.01 M phophate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	antigen ~40 kDa
species reactivity	mouse, human, rat
enhanced validation	recombinant expression
concentration	~1.5 mg/mL
application(s)	western blot: 0.5-1 µg/mL using HEK-293T cells expressing human doublecortin and rat brain extract (S1 fraction)","western blot: 1-2 µg/mL using mouse brain extract (S1 fraction)
conjugate	unconjugated
UniProt accession no.	O43602,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... DCX(1641) mouse ... Dcx(13193) rat ... Dcx(84394)

Personal Protective Equipment	Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1