Anti-Dynein Antibody, clone 74.1 ZooMAb® Mouse Monoclonal

Кат. номер

ZMS1064-25UL

ZMS1064-4X25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 74.1 is a ZooMAb® mouse recombinant monoclonal antibody that specifically detects cytoplasmic dynein.
Purified native bovine brain cytoplasmic dynein.
Anti-Dynein, clone 74.1 ZooMAb®, Cat. No. ZMS1064, is a recombinant Mouse monoclonal antibody that specifically targets Dynein and is tested for use in Flow Cytometry, Immunocytochemistry, and Western Blotting.
Quality Control Testing
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Dynein in HEK293 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Dynein in A431 and PC12 cell lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Dynein in HeLa, HepG2, and NIH 3T3 cells.
Flow Cytometry Analysis: 0.1 µg from a representative lot detected Dynein in one million HeLa cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Cytoplasmic dynein 1 intermediate chain 1 (UniProt: Q29RQ3; also known as Cytoplasmic dynein intermediate chain 1, Dynein intermediate chain 1, cytosolic, DH IC-1) is encoded by the DYNC1I1 gene (Gene ID: 613724) in bovine. Cytoplasmic dynein complexes are microtubules-associated retrograde transport motors. Cytoplasmic dynein 1 It is the more abundant motor in cells, while cytoplasmic dynein 2 takes part in intraflagellar transport. It is a multi-subunit complex of 1.5 MDa composed of a homodimer of heavy chains, two intermediate chains, light-intermediate chains, and light chains. The heavy chain homodimer acts as the dynein complex core and binds microtubules to enable ATP-dependent cytoplasmic dynein movement. The other dynein subunits associate as homodimers and play regulatory roles in maintaining the complex stability, modulating its activity, and mediating its interaction with accessory and cargo proteins. Cytoplasmic dynein 1 intermediate chain 1 acts as one of the several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are involved in linking dynein to cargos and to adapter proteins that regulate dynein function. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200,
biological source	mouse
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	74.1, monoclonal, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt ~ 68.56 kDa, observed mol wt ~78 kDa
species reactivity	rat, mouse, human
species reactivity (predicted by homology)	bovine, Drosophila, sheep, fish, Xenopus
packaging	antibody small pack of 25 µL
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	flow cytometry: suitable, immunocytochemistry: suitable, western blot: suitable
isotype	IgG2b
conjugate	unconjugated
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable