Anti-Fibroblast Growth Factor Receptor-2, Cytoplasmic antibody produced in rabbit F0300-.5ML

MDL number:	MFCD02096344
NACRES:	NA.41

Fibroblast growth factors receptors (FGFRs) are members of the tyrosine kinase family of growth factor receptors. They are glycosylated 110-150 k Da proteins that are constructed of an extracellular ligand binding region with either two or typically three immunoglobulin (Ig)-like domains and an eight amino acid ′acidic box′, a transmembrane region and a cytoplasmic split tyrosine kinase domain that is activated following ligand binding and receptor dimerization. At the mRNA level, FGFR-2 is highly expressed in developing human tissues including the brain (preferentially in glial cells), choroid plexus, skin, lung, kidney and bone. It is widely expressed in many adult human and animal tissues. It may be found in several anchorage-dependent cells, such as normal and malignant breast cancer cells.
synthetic peptide corresponding to amino acids 809-821 of the cytoplasmic region of human FGFR-2 (bek).
Anti-Fibroblast Growth Factor Receptor-2, Cytoplasmic antibody produced in rabbit was used for immunohistochemistry of free-floating rat brain tissue sections.
Anti-Fibroblast Growth Factor Receptor-2 has been used in immunoblotting, immunoprecipitation, immunohistochemistry,western blotting.
Biochem/physiol Actions
Fibroblast growth factors (FGFs) and their receptors, FGFRs, are important regulators of cell proliferation, migration, tissue repair and differentiation during vertebrate development. FGFR-2 isoforms are expressed exclusively on cells of epithelial origin and are involved in tumor progression in thyroid cancers. Abnormalities in FGFR-2 expressions have been observed in skin, bone and cancer pathologies.
Fibroblast growth factors receptors (FGFRs) mediated signal transduction requires dimerization or oligomerization and autophosphorylation of the receptors through their tyrosine kinase domain. Mutations in the extracellular domain of FGFR-2 causes Crouzon, as well as other craniosynostosis syndromes (e.g. Pfeiffer′s, Apert′s, Jackson-Weiss′), disorders of human skeletal development.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	antigen 115-125 kDa (doublet)
species reactivity	human
enhanced validation	recombinant expression
application(s)	immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2-4 µg/mL using trypsin-digested, human tissue sections","western blot: 0.25-0.5 µg/mL using extracts/lysates of FGFR-2 transfected cells
conjugate	unconjugated
UniProt accession no.	P21802,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... FGFR2(2263)

Personal Protective Equipment	Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable