Anti-Flt3 ligand Antibody, clone 5G18 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1096-4X25UL

ZRB1096-25UL

ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.Clone 5G18 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Flt3 ligand. It targets an epitope within 25 amino acids from the C-terminal, cytoplasmic domain.
KLH-conjugated linear peptide corresponding to 25 amino acids from the C-terminal, cytoplasmic domain of human Flt3 ligand.
Quality Control Testing
Evaluated by Western Blotting in Raw264.7 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Flt3 ligand in Raw264.7 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Flt3 ligand in HeLa and MCF-7 cell lysates.
Affinity Binding Assay: A representative lot of this antibody bound Flt3 ligand peptide with a KD of 1.0 x 10-12 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Flt3 ligand in MCF-7 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Flt3 ligand, clone 5G18 ZooMAb®, Cat. No. ZRB1096, is a recombinant Rabbit monoclonal antibody that specifically targets Flt3 ligand and is tested for use in Affinity Binding Assay, Immunocytochemistry, and Western Blotting.
Target description
Fms-related tyrosine kinase 3 ligand (UniProt: P49771; also known as Flt3 ligand, Flt3L, FL, SL cytokine) is encoded by the FLT3LG gene (Gene ID: 2323) in human. Flt3-ligand is an important regulator of hematopoiesis that synergizes with a number of colony-stimulating factors and interleukins. It is expressed on myeloid, lymphoid, and dendritic cell progenitors. Expression of its receptor is restricted only to hematopoietic progenitors, early B cells and myeloid cells. Binding of Flt3-ligand to its receptor (Flt3) leads to receptor homodimerization and subsequent conformational changes that result in phosphorylation of its tyrosine kinase domains. This leads to rapid activation of receptor, its internalization, downstream actions, and degradation. Administration of Flt-3 ligand to mice is shown to cause a significant expansion of both CD8 + and CD8 dendritic cells at multiple sites, including spleen, lymph nodes, thymus, liver, lung, and bone marrow. Three isoforms of Flt3 have been described that are produced by alternative splicing. Isoform one is a single-pass type I membrane protein, isoform 2 is a homodimeric secreted or extracellular protein, and isoform 3 lacks the first 82 amino acids. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Tsapogas, P., et al. (2017). Int. J. Mol. Sci. 18(6): 1115).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	rabbit
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	5G18, recombinant monoclonal
description	recombinant, expressed in HEK 293 cells
product line	ZooMAb®
form	lyophilized
mol wt	calculated mol wt 26.42 kDa","observed mol wt ~35 kDa
species reactivity	mouse, human
species reactivity (predicted by homology)	monkey
packaging	antibody small pack of 25 µL
enhanced validation	recombinant expression
application(s)	affinity binding assay: suitable","immunocytochemistry: suitable","western blot: suitable
isotype	IgG
epitope sequence	C-terminus
conjugate	unconjugated
Protein ID accession no.	NP_001450.2,
UniProt accession no.	P49771,
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable