Anti-Glucagon Antibody, clone 6D6 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1115-25UL

ZRB1115-4X25UL

ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 6D6 is a Rabbit recombinant monoclonal antibody that specifically detects glucagon.
KLH-conjugated linear peptide corresponding to full-length human glucagon.
Quality Control Testing
Evaluated by Immunohistochemistry (Paraffin) in human pancreas tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected Glucagon in human pancreas tissue sections.
Tested applications
Affinity Binding Assay: A representative lot of this antibody bound Glucagon with a KD of 1.0 x 10-12 in an affinity binding assay.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Glucagon in human pancreas tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Glucagon, clone 6D6 ZooMAb®, Cat. No. ZRB1115, is a recombinant Rabbit monoclonal antibody that targets Glucagon and is tested for use in Affinity Binding Assay, Immunofluorescence, and Immunohistochemistry (Paraffin).
Target description
Pro-glucagon (UniProt: P01275) is encoded by the GCG gene (Gene ID: 2641) in human. Pro-glucagon is a 180 amino acid peptide that is post-translationally processed in a tissue-specific manner in pancreatic alpha cells and intestinal L cells. In pancreatic alpha cells, glucagon (aa 53-81) is produced by cleavage of pro-glucagon by proprotein Convertase Subtilisin/Kexin Type 2 (PCSK2/PC2). It is secreted in response to low levels of blood glucose. Hence, its circulating levels are low in the prandial state and increase 2 3 fold in response to fasting or hypoglycemia. Its actions are mediated via the glucagon receptor (GCGR), a G protein-coupled receptor that is most abundantly expressed in the liver. Traces of GCGR are also reported in several extrahepatic tissues, such as the brain, heart, kidney, gastrointestinal tract, and adipose tissues. It regulates blood glucose levels by increasing gluconeogenesis and reducing glycolysis. As a counterregulatory hormone of insulin, glucagon raises plasma glucose levels in response to insulin-induced hypoglycemia. In diabetic subjects, it plays a role in initiating and maintaining hyperglycemic condition. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	rabbit
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	6D6, recombinant monoclonal
description	recombinant, expressed in HEK 293 cells
product line	ZooMAb®
form	lyophilized
mol wt	calculated mol wt 3.49 kDa
purified by	using Protein A
species reactivity	human
species reactivity (predicted by homology)	mouse, rat
packaging	antibody small pack of 25 µL
enhanced validation	recombinant expression
application(s)	affinity binding assay: suitable","immunofluorescence: suitable","immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
isotype	IgG
epitope sequence	Unknown
conjugate	unconjugated
Protein ID accession no.	NP_002045.1,
UniProt accession no.	P01275,
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable