Anti-GLUT-4 Antibody, clone 1M13 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1240-4X25UL

ZRB1240-25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 1M13 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Glucose transporter type 4. It targets an epitope within 13 amino acids from the C-terminal region.
KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal region of human Glucose transporter type 4 (GLUT-4).
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GLUT-4 in HeLa cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GLUT-4 in NIH3T3, HepG2, and L6 cell lysates.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected GLUT-4 in HeLa, A431, HepG2, and NIH 3T3 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected GLUT-4 in human heart tissue sections.
Flow Cytometry Analysis: 0.1 µg from a representative lot detected GLUT-4 in one million HeLa cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-GLUT-4;recombinant Rabbit monoclonal antibody that specifically targets GLUT-4 and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Target description
Solute carrier family 2, facilitated glucose transporter member 4 (UniProt: P14672; also known as Glucose transporter type 4, insulin-responsive, GLUT-4) is encoded by the SLC2A4 (also known as GLUT4) gene (Gene ID: 6517) in human. Glucose transport in mammalian cells depends upon membrane-associated carrier proteins. Mammalian cells possess two types of glucose carriers (a) the Na+-glucose co-transporter and the (b) facilitative glucose transporter. The Na+-glucose co-transporter transports glucose against its concentration gradient by coupling its uptake with the uptake of Na+. It is largely expressed in epithelial cells of the small intestine and kidney. The facilitative glucose carriers of the GLUT family accelerate the transport of glucose down its concentration gradient by facilitative diffusion. Members of the GLUT family are expressed in a tissue- and cell-specific manner and exhibit distinct kinetic and regulatory properties that reflect their specific functional roles. GLUT4 is a multi-pass membrane protein with 6 extracellular domains, 12 transmembrane domains, and 7 cytoplasmic domains. It is expressed in insulin sensitive tissues, such as muscle and adipose tissue and its levels change in response to insulin. It is responsible for increased glucose disposal in these tissues in the postprandial state and is vital in whole-body glucose homeostasis. In the absence of insulin, it is efficiently retained intracellularly within storage compartments in muscle and fat cells. However, upon insulin stimulation, it translocates from these compartments to the cell surface where it transports glucose from the extracellular milieu into the cell. GLUT-4 contains a dileucine internalization motif (aa 489-490) that is critical for its intracellular sequestration. Mutations in SLC2A4 gene are known to cause non-insulin-dependent diabetes mellitus, a multifactorial disorder of glucose homeostasis. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200,
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	1M13, monoclonal, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt ~ 54.79 kDa, observed mol wt ~ 53 kDa
species reactivity	mouse, rat, human
species reactivity (predicted by homology)	monkey, bovine
packaging	antibody small pack of 25 µL
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable