Anti-Green Fluorescent Protein Antibody, clone 4I10, ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1097-25UL

ZRB1097-4X25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.,
Specificity
Clone 4I10 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Green fluorescent protein (GFP) from Aequorea victoria (Jelly Fish).
His-tagged full-length recombinant Green fluorescent protein (GFP) from Aequorea victoria (Jelly fish).
Immunoprecipitation Analysis: A 1:1,000 dilution from a representative lot immunoprecipitated Green Fluorescent Protein in GFP spiked into a HeLa cell lysate.
Immunofluorescence Analysis: A 1:250 dilution from a representative lot detected Green Fluorescent Protein in hGFAP-GFP transgenic mouse brain tissue sections.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Green Fluorescent Protein in membranes of HEK 293T cells transfected with GFP.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Anti-Green Fluorescent Protein, clone 4I10 ZooMAb , Cat. No. ZRB1097, is a highly specific recombinant rabbit monoclonal antibody that targets Green Fluorescent Protein and has been tested for use in Immunocytochemistry, Immunofluorescence, and Western Blotting.
Target description
Green fluorescent protein (UniProt: P42212; also known as GFP) is encoded by the GFP gene in Aequorea victoria (Jelly Fish). GFP is a 27 kDa protein from jellyfish that serves as an energy-transfer acceptor. It transduces the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer and it fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin. GFP has an absorption max at 395 nm and the emission peaks at 507 to 510 nm. However, the exact value of emission max depends on the environment of the chromophore. GFP is an important tool in cell biology research that displays stability under fixation conditions and is suitable for a variety of applications, such as tracing cell lineage tracer, reporter of gene expression, or exploring protein-protein interactions. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit (recombinant)
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	4I10, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt 26.89 kDa, observed mol wt ~30 kDa
species reactivity	all
packaging	antibody small pack of 25 µL
greener alternative product characteristics	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry,.
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	immunocytochemistry: suitable, immunofluorescence: suitable, western blot: suitable (peptide)
isotype	IgG
conjugate	unconjugated
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable