Anti-Histone Deacetylase 4 (HDAC4) (DM-15) antibody produced in rabbit H9536-200UL

MDL number:	MFCD03791600
NACRES:	NA.41

Histone deacetylases (HDACs) are competing enzymes, belonging to histone deacetylase family. There are two classes of HDACs with six to seven different types of HDACs proteins. HDAC1,HDAC2 and HDAC3 belong to Class I HDACs and HDAC4, HDAC6, and HDAC7 belong to Class II HDACs. Class I HDACs consists of a single deacetylase domain at the N-termini and diversified C-terminal regions, while Class II contains a deacetylase domain at C-terminal position.
Mammalian histone deacetylases (HDACs) are classified into three classes according to sequence homology. HDAC1, HDAC2, HDAC3 and HDAC8 belong to class I HDACs and HDAC4, HDAC6, and HDAC7 belong to class II HDACs. Class III consists of the yeast sirtuins (sir2-like protein). Class I HDACs are ubiquitously expressed. Whereas, most of the class II HDACs show tissue-specific expression. The antibody is affinity-purified using the immunizing peptide immobilized on agarose.
Specificity
Anti-Histone Deacetylase 4 (HDAC4), recognizes human, mouse and rat HDAC4.
Synthetic peptide corresponding to amino acid residues of human HDAC4 with C-terminal added lysine conjugated to KLH.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below:

• Western Blotting
• Immnofluorescence
• Immunoprecipitation

Biochem/physiol Actions
Histone deacetylase 4 (HDAC4) contains import and export nuclear signal that aids in nucleocytoplasmic shuttling. It has physical and functional interactions with transcription factor myocyte enhancer factor-2 (Mef2) protein and 14-3-3. This interaction disturbs nucleocytoplasmic shuttling and then guides HDAC4 to the cytoplasm and the nucleus, respectively. In addition, HDAC4 with HDAC5 and HDAC7 represses transcription by binding to the nuclear receptor co-repressors silencing mediator of retinoic acid and thyroid hormone receptors (SMRT) and nuclear receptor co-repressor (NCOR).
Studies show that HDAC4 contains a composed import and export nuclear signal that aids in nucleocytoplasmic shuttling. It has physical and functional interactions with transcription factor MEF2C and 14-3-3. This interaction results in the shuttling and it guides HDAC4 to the cytoplasm and the nucleus.The C-terminal domain of HDAC4 shows innate histone deacetylase activity. It is seen that MEF2C combines with HDAC4 at the N-terminal domain and downmodulate c-jun promoter activity.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8°C for up to one month. For prolonged storage, freeze in working aliquots at -20°C. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	antigen ~140 kDa
species reactivity	rat, mouse, human
enhanced validation	recombinant expression
application(s)	immunoprecipitation (IP): 10-20 µg using RIPA extract of HeLa nuclei","indirect immunofluorescence: 1:250 using HEK 293T cells expressing recombinant mouse HDAC4","microarray: suitable","western blot: 1:1,000 using whole extracts of mouse NIH3T3 cells","western blot: 1:500 using whole extracts of rat brain
conjugate	unconjugated
UniProt accession no.	P56524,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... HDAC4(9759) mouse ... Hdac4(208727) rat ... Hdac4(363287)

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	Not applicable
Flash Point C	Not applicable