Anti-Histone Deacetylase 8 (HDAC8) antibody produced in rabbit H8038-200UL

MDL number:

MFCD04975119

Histone deacetylases (HDACs) are competing enzymes, belonging to histone deacetylase family. There are two classes of HDACs with six to seven different types of HDACs proteins. HDAC1,HDAC2, HDAC3, and HDAC8 belong to Class I HDACs and HDAC4, HDAC6, HDAC7, HDAC9, and HDAC10 belong to Class II HDACs. Class I HDACs consists of a single deacetylase domain at the N-termini and diversified C-terminal regions, while Class II contains a deacetylase domain at C-terminal position. Studies show that HDAC8 is a sex-linked gene located on the chromosome at position Xq21.2 - q21.3. HDAC8 gene has a molecular weight of 43kDa and it encodes a 377 amino acid protein. It is present within the nucleas. HDAC8 mRNA is seen in heart, lung, kidney, and pancreas.
The antibody is affinity-purified using the immunizing peptide immobilized on agarose.
synthetic peptide corresponding to amino acid residues 352-370 of human HDAC8, conjugated to KLH. The corresponding sequence in mouse differs by one amino acid.
Anti-Histone Deacetylase 8 (HDAC8) antibody produced in rabbit has been used in western blotting, immunohistofluorescence and immunoblotting.
Biochem/physiol Actions
Histone deacetylation results in transcription repression leading to the formation of tight nucleosomal structure which prevents DNA accessing. {5} HDAC8 controls HDAC activity on H4 histone peptide substrates. HDAC8 is similar to the HDAC class I enzymes. In vitro expression and activity of HDAC8 was examined using FLAG tagged- HDAC8 and HDAC1. These were transfected into HeLa cells and Sf9 insect cells. After the immunoprecipitation of cell lysates the expression results were confirmend using Western blotting. Studies show that HDAC8 may play a role in role in transcriptional regulation and could possibly be regulated in a temporal or compartment-specific manner.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	antigen ~43 kDa
species reactivity	human, mouse
enhanced validation	recombinant expression
application(s)	immunocytochemistry: 1-2 µg/mL using cultured 293T cells expressing recombinant human HDAC8","immunoprecipitation (IP): 1-2 µg using extract of 293T cells expressing recombinant human HDAC8","western blot: 2-4 µg/mL using whole extract of human colon adenocarcinoma SW620 cells and a chemiluminescent detection reagent
conjugate	unconjugated
UniProt accession no.	Q9BY41,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... HDAC8(55869) mouse ... Hdac8(70315)

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point F	Not applicable
Flash Point C	Not applicable