Anti-KCNQ2 Antibody, clone 2G18 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1299-25UL

ZRB1299-4X25UL

ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.Clone 2G18 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Potassium voltage-gated channel subfamily KQT member 2 (KCNQ2). It targets an epitope within 20 amino acids from the C-terminal, cytoplasmic domain.
KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal, cytoplasmic domain of human Potassium voltage-gated channel subfamily KQT member 2 (KCNQ2).
Anti-KCNQ2, clone 2G18 ZooMAb®, Cat. No. ZRB1299, is recombinant Rabbit monoclonal antibody that detects KCNQ2 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Quality Control Testing
Evaluated by Western Blotting in Mouse brain microsomal preparation.
Western Blotting Analysis: 1:10,000 of this antibody detected KCNQ2 in Mouse brain microsomal preparation.

Tested applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected KCNQ2 in human brain tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound KCNQ2 with a KD of 1.0 x 10-7 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected KCNQ2 in E18 rat cortical cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Potassium voltage-gated channel subfamily KQT member 2 (UniProt: O43526; also known as KQT-like 2, Neuroblastoma-specific potassium channel subunit alpha KvLQT2, Voltage-gated potassium channel subunit Kv7.2, KCNQ2) is encoded by the KCNQ2 gene (Gene ID: 3785) in human. KCNQ2 is a multi-pass membrane protein with four cytoplasmic domains, six transmembrane domains, and four extracellular domains. It is highly expressed in areas containing neuronal cell bodies and low expression is observed in spinal cord and corpus callosum. KCNQ2 associates with KNNQ3 to form a potassium channel with essentially identical properties to the channel underlying the native M-current, a slowly activating and deactivating potassium conductance, which plays a role in determining the subthreshold electrical excitability of neurons and the responsiveness to synaptic inputs. It is also important in regulation of neuronal excitability. Mutations in KCNQ2 gene are usually inherited in an autosomal-dominant manner in epileptic syndromes and KCNQ2-associated seizures are observed as early as the first week after birth. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Lee, IC., et al. (2020). Sci. Rep. 10; Article 13375).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	rabbit
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	2G18, recombinant monoclonal
description	recombinant, expressed in HEK 293 cells
product line	ZooMAb®
form	lyophilized
mol wt	calculated mol wt 95.85 kDa","observed mol wt ~150 kDa
species reactivity	mouse, human, rat
packaging	antibody small pack of 25 µL
enhanced validation	recombinant expression
application(s)	affinity binding assay: suitable","immunocytochemistry: suitable","immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable","western blot: suitable
isotype	IgG
epitope sequence	C-terminal
conjugate	unconjugated
Protein ID accession no.	NP_742105,
UniProt accession no.	O43526,
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable