Anti-Laminin antibody, Mouse monoclonal SAB4200719-100UL

NACRES:

NA.41

Monoclonal anti-laminin antibody (mouse IgG1 isotype) is derived from the LAM-89 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified laminin from human placenta. Monoclonal anti-laminin antibody specifically recognizes laminin from human, feline and porcine origin. The antibody shows reactivity with laminin and specifically staining basal membrane of blood vessels, epithelium, nerve and muscle fiber. Monoclonal anti-laminin shows no reaction with collagen IV, fibronectin, vitronectin and chondroitin sulfate type A, B or C. Laminins are complexed extracellular glycoproteins which assemble the basal components of the basement membrane (BM). Each laminin is a heterotrimer (~850kDa) comprising of an α (~400kDa), β (~200kDa) and γ (~200kDa) chains all held together by disulfide bonds.
Specificity
Monoclonal Anti-Laminin antibody specifically recognizes Laminin from human, feline and porcine origin.
purified Laminin from human Placenta
Monoclonal Anti-Laminin is recommended to use in various immunochemical assays, including immunohistochemistry, immunoblotting (~850 kDa), immunofluorescence, Enzyme linked immunosorbent assay (ELISA) and electron microscopy.
Biochem/physiol Actions
Laminins contribute to the structure of the extracellular matrix (ECM). It also plays a major role in the regulation of variety of cellular processes, such as the rates of cell proliferation, differentiation, adhesion, migration, cell shaping, phenotype stability and resistance to apoptosis. Laminin polymers forms supramolecular network with collagen IV polymers through heparan sulfate proteoglycan and by nidogen (entactin) and plays an important role in the basement membrane (BM) stability. In mouse, mutations in the gene is associated with the development of muscular dystrophy.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at –20°C. For continuous use, store at 2–8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	mouse
antibody form	purified from hybridoma cell culture
antibody product type	primary antibodies
clone	LAM-89, monoclonal
form	buffered aqueous solution
mol wt	~850 kDa
species reactivity	feline, porcine, human
enhanced validation	independent
concentration	~1.0 mg/mL
application(s)	ELISA: suitable","electron microscopy: suitable","immunoblotting: 0.03-0.06 µg/mL using Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane","immunofluorescence: suitable","immunohistochemistry: 20-40 µg/mL using pronase-retrieved formalin-fixed, paraffin-embedded human tongue sections.
isotype	IgG1
UniProt accession no.	P25391,
shipped in	dry ice
storage temp.	20°C

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable