Anti-Laminin antibody produced in rabbit

MDL number:	MFCD00162586
NACRES:	NA.41

Кат. номер

L9393-.2ML

L9393-100UL

L9393-.5ML

Laminin is a ubiquitous non-collagenous connective tissue glycoprotein that is a major constituent of basement membranes. Laminins are extracellular matrix proteins. The LAMA1 (laminin subunit α 1) gene is mapped to human chromosome 18p11. The antibody is isolated from antiserum by immunospecific methods of purification. Antigen specific isolation removes essentially all rabbit serum proteins, including immunoglobulins that do not specifically bind to laminin.
Specificity
Specificity of the anti-laminin antibodies is determined by indirect immunofluorescent labeling of formalin-fixed, paraffin-embedded human or animal tissue sections, and by dot blot immunoassay. By indirect immunofluorescence the antibody demonstrates specific basement membrane staining of enzymatically unmasked human and animal tissue. In the dot blot immunoassay the rabbit anti-laminin antibody reacts with laminin but not with fibronectin, vitronectin, collagen IV, or chondroitin sulfate types A, B, and C. The affinity isolated antibody to laminin will react with laminin of human, mammal, avian, reptilian, and amphibian sources.
Laminin isolated from the basement membrane of Englebreth Holm-Swarm (EHS) mouse sarcoma.
Anti-Laminin antibody has been used in immunohistochemical staining and immunohistochemistry.
Rabbit Anti-Laminin antibody may be used in immunohistochemistry for marking blood vessel walls in different species, classification of various disease processes involving basement membranes, identification of the origin of human tumors and their classification, and for distinguishing between non-invasive and invasive lesions. The antibody may be used to monitor levels of laminin in biological fluids and for experimental production of basement membrane lesions in vivo.
A working dilution of at least 1:1,000 was determined by a dot blot immunoassay using laminin at 50 ng per dot.
A working dilution of at least 1:25 was determined by indirect immunohistology using formalin-fixed, paraffin-embedded human and animal tissues.
Biochem/physiol Actions
LAMA1 (laminin subunit α 1) is involved in several diseases like cancer, infections, inflammatory diseases and autoimmune disorders. Mutations in LAMA1 result in cerebellar dysplasia and cysts with and without retinal dystrophy. LAMA1 is also essential in the assembly of basement membrane and early growth of embryo.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	300
biological source	rabbit
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
species reactivity	human, animal
enhanced validation	independent
concentration	0.5 mg/mL
application(s)	dot blot: 1:1,000","immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:30 using human and animal tissues","microarray: suitable
conjugate	unconjugated
Featured Industry	Research Pathology
UniProt accession no.	P25391,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... LAMA1(284217) mouse ... Lama1(16772)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable