Anti-LHPP Antibody, clone 3B18 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1050-25UL

ZRB1050-4X25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.,
Specificity
Clone 3B18 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Phospholysine phosphohistidine inorganic pyrophosphate phosphatase (LHPP).
His-tagged full-length recombinant human Phospholysine phosphohistidine inorganic pyrophosphate phosphatase (LHPP).
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LHPP in lysates from rat kidney, human liver, and COS-7 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected LHPP in A431 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected LHPP in human liver tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound human recombinant LHPP with a KD of 8.6 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-LHPP, clone 3B18 ZooMAb, Cat. No. ZRB1050, is a recombinant Rabbit monoclonal antibody that specifically targets LHHP and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Target description
Phospholysine phosphohistidine inorganic pyrophosphate phosphatase (UniProt: Q9H008; also known as EC:3.6.1.1, Hlhpp, LHPP) is encoded by the LHPP gene (Gene ID: 64077) in human. LHPP is an evolutionarily conserved member of the HAD-like hydrolase superfamily with phosphatase activity. It displays broad substrate specificity and can hydrolyze imidodiphosphate, 3-phosphohistidine, and 6-phospholysine. It can also hydrolyze inorganic diphosphate, but with reduced efficiency. It has two substrate binding sites that are located in amino acids 17-19 and 54-55. Its higher expression has been reported in brain, and somewhat lower expression in liver and kidney. LHHP expression is shown to be down regulated in liver and bladder cancers and knockdown of LHPP promotes the proliferation and growth of tumor cells. Conversely, sustained hepatic expression of LHPP in a murine model of hepatocellular carcinoma is reported to reduce tumor burden and prevent the loss of liver function. Silencing of LHHP can lead to enhanced phosphorylation of Akt and p65 and up-regulates the level of Bcl-2 and cyclin D1. Two isoforms of LHPP have been described that are produced by alternative splicing. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Hindupur, SK., et al. (2018). Nature. 555(7698); 678-682; Li, Y., et al. (2019). Biosci Rep. 39(7); BSR20182270.).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit (recombinant)
Quality Level	200,
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	3B18, monoclonal, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt 29.16 kDa, observed mol wt ~30 kDa
species reactivity	human, rat, monkey
packaging	antibody small pack of 25 µL
greener alternative product characteristics	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry,.
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	affinity binding assay: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
greener alternative category	Aligned,
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable