Anti-Lysyl Oxidase (C-terminal) antibody produced in rabbit

NACRES:

NA.41

Кат. номер

L4669-200UL

L4669-25UL

The LOX gene is located on the human chromosome at 5q23.1.
The gene LOX (lysyl oxidase) encodes a copper-dependent amine oxidase that belongs to LOX family of proteins. These proteins contain highly conserved C- terminal mature catalytic domains that include the copper binding site, the lysyl tyrosyl quinine (LTQ) cofactor residues, and the cytokine receptor like (CRL) domain. It is present in both intercellular and intracellular locations.
Specificity
Anti-Lysyl Oxidase (C-terminal) specifically recognizes human lysyl oxidase (LOX).
Anti-Lysyl Oxidase (C-terminal) antibody produced in rabbit has been used in:

• western blotting
• immunofluorescence staining
• immunohistochemistry

Biochem/physiol Actions
The gene LOX (lysyl oxidase) encodes an extracellular matrix remodeling enzyme that catalyzes the oxidation of primary amino group of peptidyl lysine to reactive peptidyl aldehydes. It is mainly involved in the oxidation of lysine residues in elastin and collagen, resulting in the formation of covalent cross-linkages, which stabilize these fibrous proteins. It also plays a role in several cellular processes such as developmental regulation, tumor suppression, cell motility, and cellular senescence. It is involved in hypoxia-induced metastasis and serves as a therapeutic target for the treatment of metastases.
Altered LOX gene expression and activity are linked to skin aging and senescence. Decreased LOX gene expression and activity have been associated with severe connective tissue disorders such as Ehler-Danlos syndrome, cutis laxa, and Menkes′ syndrome. Increased LOX gene expression are associated with the development of fibrotic diseases that involve connective tissue remodeling, such as atherosclerosis, scleroderma and liver cirrhosis. LOX protein also plays an important role in breast cancer metastasis.
LOX is implicated in various intracellular functions such as the regulation of cellular differentiation, cell migration and gene transcription.
Physical form
Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	antigen ~50 kDa
species reactivity	human
enhanced validation	recombinant expression
concentration	~1 mg/mL
application(s)	western blot: 1.5-3.0 mg/mL using HEK-293T cellls expressing human LOX
conjugate	unconjugated
UniProt accession no.	P28300,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... LOX(4015) mouse ... Lox(16948) rat ... Lox(24914)

Personal Protective Equipment	Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US),
RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable