Anti-MAP2 Antibody, clone AP20 ZooMAb® Mouse Monoclonal

Кат. номер

ZMS1013-4X25UL

ZMS1013-25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.,
Specificity
Clone AP20 is a is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects MAP2.
Full-length purified microtubule protein (MAP2) from bovine brain.
Anti-MAP2, clone AP20 ZooMAb, Cat. No. ZMS1013, is a recombinant Mouse monoclonal antibody that specifically targets MAP2 and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MAP2 in mouse and rat brain tissue lysates.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MAP2 in SH-SY5Y cell line and E18 cortical neurons.
Immunohistochemistry (Paraffin) Analysis: A 1:10-100 dilution from a representative lot detected MAP2 in mouse brain and rat brain tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Microtubule-associated protein (UniProt: F1MEW3; also known as MAP2) is encoded by the MAP2 gene (Gene ID: 281294) in bovine. MAP2 is a neuron-specific protein that stabilizes microtubules in the dendrites of postmitotic neurons. Its major function is to stabilize neuronal shape by promoting microtubule synthesis and cross-linking with other components of the cytoskeleton. Phosphorylation of MAP2 controls its association with the cytoskeleton and it is developmentally regulated. MAP2 can undergo phosphorylation by GSK-3 and p44/42 MAP kinase. Phosphorylation of MAP2 at threonine 1620 and 1623 by GSK-3 are reported to inhibits MAP2 association with microtubules and affect microtubule stability. MAP2 consists of at least three isoforms produced from a single gene: high molecular mass MAP2a and MAP2b, and low molecular mass MAP2c that is also known as the juvenile MAP2c, which is expressed only during early development and exists mainly in axons. MAP2a appears to replace MAP2c during growth and MAP2b is expressed throughout life. MAP2 can also serve as a marker of synaptic plasticity and its levels are observed to increase after traumatic brain injury. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Soltani, MH., et al. (2005). Am. J. Pathol. 166(6); 1841-1850).
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200,
biological source	mouse
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	AP20, monoclonal, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt 217 kDa, observed mol wt ~260 kDa
species reactivity	human, rat, mouse
species reactivity (predicted by homology)	chicken, Xenopus, bovine, quail
packaging	antibody small pack of 25 µL
greener alternative product characteristics	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry,.
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype	IgG1
conjugate	unconjugated
greener alternative category	Aligned,
shipped in	ambient
storage temp.	2-8°C

WGK Germany

WGK 1