Anti-Mitofusin-2 Antibody, clone 2D19 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1202-25UL

ZRB1202-4X25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 2D19 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Mitofusin-2. It targets an epitope within 21 amino acids from the cytoplasmic domain in the C-terminal half.
KLH-conjugated linear peptide corresponding to 21 amino acids from the first cytoplasmic domain in the C-terminal region of human Mitofusin-2.
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Mitofusin-2 in HeLa cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Mitofusin-2 in U2OS, COS-7, and PC12 cell lysates.
Immunocytochemistry Analysis: A 1:100 dilution from a representative detected Mitofusin-2 in HeLa cells.
Affinity Binding Assay: A representative lot of this antibody bound Mitofusin-2 peptides with a KD of 1.4 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Mitofusin-2, clone 2D19 ZooMAb®, Cat. No. ZRB1202, is a recombinant Rabbit monoclonal antibody that specifically targets Mitofusin-2 and is tested for use in Affinity Binding Assay, Immunocytochemistry, and Western Blotting.
Target description
Mitofusin-2 (UniProt: O95140; also known as Transmembrane GTPase MFN2) is encoded by the MFN2 (also known as CPRP1, KIAA0214) gene (Gene ID: 9927) in human. Mitofusin-1 and 2 are highly homologous mitochondrial proteins that display ~80% similarity in humans. Mitofusin-2 is a multi-pass membrane protein that serves as a mitochondrial outer membrane GTPase to mediate mitochondrial clustering and fusion. It is ubiquitously expressed at low level, but higher expression is observed in heart and kidney. It consists of a large, cytoplasmic N-terminal GTPase domain, followed by a spacer, a first coiled-coil heptad-repeat (HR1) domain, a spacer, two transmembrane domains that cross the outer mitochondrial membrane, a spacer and a second, C-terminal heptad-repeat domain (HR2). Between HR1 and transmembrane domains, it contains a proline-rich domain that participates in protein protein interactions. Mitofusin-2 plays a key role in mitochondrial metabolism and is involved in mitochondrial fusion, trafficking, turnover, contacts with other organelles, and in the clearance of damaged mitochondria via selective mitophagy. Mitofusin-2 is phosphorylated by PINK1 and the phosphorylated form functions as a receptor for parkin that mediates its ubiquitination, a signal to mark damaged mitochondria. It is also required in the control of unfolded protein response upon endoplasmic reticulum stress. Mitofusion-2 knockout cells display mitochondrial spheres or ovals of different sizes and are several folds larger in diameter than wild-type. Mutations in MFN2 gene are known to cause Charcot-Marie-Tooth disease that is characterized by signs of axonal degeneration and progressive distal muscle weakness and atrophy. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Filadi, R., et al. (2018). Cell Death Dis. 9; Article 330).
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200,
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	2D19, monoclonal, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt ~ 86.4 kDa, observed mol wt ~ 79 kDa
species reactivity	rat, human, monkey
packaging	antibody small pack of 25 µL
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	affinity binding assay: suitable, immunocytochemistry: suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable