Anti-p-Ubiquitin (pSer65) Antibody, clone 2N21 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1494-25UL

ZRB1494-4X25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.,
Specificity
Clone 2N21 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects ubiquitin phosphorylated on serine 65.
KLH-conjugated linear peptide corresponding to 13 amino acids from the ubiquitin-like 1 domain of human polyubiquitin phosphorylated on serine 65.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected p-Ubiquitin (pSer65) in lysate from HeLa cells transfected with PINK and treated with CCCP.
Flow Cytometry Analysis: 0.1 µg from a representative lot detected p-Ubiquitin (pSer65) in one million PC-3 cells treated with CCCP.
Peptide Inhibition Assay: Target band detection in lysate from HeLa cells transfected with PINK and treated with CCCP (15 mM, 5 h) was prevented by preblocking of a representative lot with the phosphorylated Ser 65 ubiquitin peptide, but not by corresponding non-phosphopeptide.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected p-Ubiquitin (pSer65) in HeLa cells transfected with PINK and treated wtih CCCP.
Affinity Binding Assay: A representative lot of this antibody bound phosphorylated serine 65 Ubiquitin peptide with a KD of 2.5 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-p-Ubiquitin (pSer65), clone 2N21 ZooMAb, Cat. No. ZRB1494, is a recombinant Rabbit monoclonal antibody to detect ubiquitin (pSer65) in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Peptide Inhibition Assay, and Western Blotting.
Target description
BDNF/NT-3 growth factors receptor (UniProt: Q16620; also known as EC:2.7.10.1, GP145-TrkB, Trk-B, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, Tropomyosin-related kinase B) is encoded by the NTRK2 (also known as TRKB) gene (Gene ID: 4915) in human. TrkB is a single-pass type I membrane protein that is internalized to endosomes upon ligand-binding. It is synthesized with a signal peptide (aa 1-31) that is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 32-430), a transmembrane domain (aa 431-454), and a cytoplasmic domain (aa 455-822). TrkB is a receptor tyrosine kinase that is involved in the development and maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. In the central nervous system, its expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophthalmic nerve, the vestibula, and multiple facial structures. Upon ligand-binding, it undergoes homodimerization, autophosphorylation, and activation. It can then recruit, phosphorylate and/or activate several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. TrkB is also reported to play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia. Following binding of BDNF to TrkB receptor, Tyrosine 816 is phosphorylated, which allows the binding of phospholipase C gamma and activation of signaling cascade. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
0.03 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit (recombinant)
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	2N21, monoclonal, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
species reactivity	human
species reactivity (predicted by homology)	mouse, rat
packaging	antibody small pack of 25 µL
greener alternative product characteristics	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry,.
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	affinity binding assay: suitable, flow cytometry: suitable, immunocytochemistry: suitable, inhibition assay: suitable (peptide), western blot: suitable
isotype	IgG
conjugate	unconjugated
greener alternative category	Aligned,
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable