Anti-p16-INK4a Antibody, clone 5F22 Antibody, ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1437-4X25UL

ZRB1437-25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.,
Specificity
Clone 5F22 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Cyclin-dependent kinase inhibitor 2A (p16-INK4a). It targets an epitope within the first 54 amino acids from the N-terminal.
GST/His-tagged recombinant fragment corresponding to the first 54 amino acids from human Cyclin-dependent kinase inhibitor 2A (p16-INK4a).
Anti-p16-INK4a, clone 5F22, ZooMAb, Cat. No. ZRB1437, is a recombinant Rabbit monoclonal antibody that specifically targets p16-INK4a and is tested in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected p16-INK4a in HeLa, A431, HUVEC, and NIH3T3 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected p16-INK4a in human cervical cancer tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound recombinant p16-INK4a protein with a KD of 4.5 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Cyclin-dependent kinase inhibitor 2A (UniProt: P42771; also known as Cyclin-dependent kinase 4 inhibitor A, CDK4I, Multiple tumor suppressor 1, MTS-1, p16-INK4a, p16-INK4, p16INK4A) is encoded by the CDKN2A (also known as CDKN2, MTS1) gene (Gene ID: 1029) in human. p16-INK4 is a widely distributed protein that acts as a negative regulator of proliferation of normal cells. It is reported to be the principal member of the IINK4 family of CDK inhibitors. It heterodimerizes with CDK4 or CDK6 and inhibits their ability to interact with cyclin D and to phosphorylate the retinoblastoma protein. It is considered a tumor suppressor protein because of physiological role and down-regulated expression in a large number of tumors. Overexpression of p16-INK4a has also been described in several tumors, including endometrial, colorectal and basal cell carcinoma. Six isoforms of p16-INK4a have been described that are produced by alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
0.3 mg/mL after reconstitution at 25μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommended storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt 16.53 kDa, observed mol wt ~16 kDa
species reactivity	human, mouse
packaging	pkg of 25 µL
greener alternative product characteristics	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry,.
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	affinity binding assay: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
greener alternative category	Aligned,
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable