Anti-Pancreatic Polypeptide Antibody, clone 3H5, ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB939-25UL

ZRB939-4X25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Clone 3H5 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Pancreatic Polypeptide.
KLH-conjugated linear peptide corresponding to full-length human Pancreatic Polypeptide (without the signal and propeptides).
Anti-Pancreatic Polypeptide, clone 3H5 ZooMAb, Cat. No. ZRB939, is a recombinant Rabbit Monoclonal Antibody that detects pancreatic polypeptide and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunofluorescence, and Western Blotting.
Immunofluorescence Analysis: A 1:400 dilution from a representative lot detected Pancreatic Polypeptide in human pancreas tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:400 dilution from a representative lot detected Pancreatic Polypeptide in human pancreas tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Pancreatic Polypeptide in TGP52 cell line.
Target description
Pancreatic polypeptide (UniProt: P01298; also known as PP, Obinepitide, Pancreatic prohormone) is encoded by the PPY (also known as PNP) gene (Gene ID: 5539) in human. Pancreatic polypeptide is a member of the neuropeptide Y family and is secreted protein that is produced by PP cells in the pancreatic islets of Langerhans. The pancreatic prohormone is synthesized with a signal peptide (aa 1-29) and a propeptide (aa 89-95) that are subsequently cleaved off. The remaining part is cleaved off into pancreatic hormone (aa 30-65) and pancreatic icosapeptide (69-88). Pancreatic hormone acts as a regulator of pancreatic and gastrointestinal functions. Two isoforms of pancreatic polypeptide have been described that are generated by alternative splicing. Pancreatic polypeptide release is functionally associated with increased satiety, delayed gastric emptying, inhibition of gallbladder motility, and regulation of energy expenditure. Its deficiency is observed in cases of pancreatic resection and chronic pancreatitis and is associated with significant reduction in PP cell mass and abnormally high hepatic glucose production even in the presence of endogenous insulin. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Maxwell, J et al. (2014). Pancreas 43(4); 651-656).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
120 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit (recombinant)
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	3H5, monoclonal","recombinant monoclonal
product line	ZooMAb®
form	lyophilized
mol wt	calculated mol wt 10.44 kDa
species reactivity	human
species reactivity (predicted by homology)	bovine
packaging	antibody small pack of 25 µL
greener alternative product characteristics	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency.
enhanced validation	recombinant expression
application(s)	immunocytochemistry: suitable using 1:100","immunofluorescence: 1:100-1:400","immunofluorescence: suitable","immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:400","western blot: suitable using 1:1,000
isotype	IgG
conjugate	unconjugated
UniProt accession no.	P01298,
shipped in	ambient
storage temp.	2-8°C
Gene Information	human ... PPY;PNP(5539)

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable