Anti-Parvalbumin Antibody, clone 4G3 , ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1218-4X25UL

ZRB1218-25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.,
Specificity
Clone 4G3 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Parvalbumin.
GST/His-tagged full-length recombinant human Parvalbumin.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Parvalbumin in rat and human brain tissue lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Parvalbumin in human cerebellum and mouse cerebellum tissue sections.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Parvalbumin in mouse cerebellum tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Parvalbumin in PC12 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens and experimental conditions may vary.
Anti-Parvalbumin, clone 4G3 ZooMAb, Cat. No. ZRB1218, is a highly specific rabbit recombinant monoclonal antibody that targets Parvalbumin and has been tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
Target description
Parvalbumin alpha (UniProt: P20472; also known as PV) is encoded by the PVALB gene (Gene ID: 5816) in human. Parvalbumin is a low molecular weight soluble protein that binds two calcium ions with high affinity and is implicated in intracellular calcium regulation and trafficking. It is composed almost entirely of two EF-hand domains (aa. 39-74 & 78-110) and is structurally related to calmodulin and troponin C. It serves as a slow Ca2+ buffer that affects the amplitude and time course of intracellular Ca2+ transients in terminals after an action potential, and hence may regulate short-term synaptic plasticity. During nervous system development, its expression is shown to accompany the maturation of functional circuits. Parvalbumin containing neurons are abundant in the cortex, the hippocampus, and the thalamus. It is present in GABAergic interneurons in the nervous system, especially the reticular thalamus. Parvalbumin interneurons are a subset of inhibitory GABAergic neurons that control the excitability of post-synaptic pyramidal neurons. Dysfunction or loss of parvalbumin interneurons has been implicated in cognitive deficits associated with several well-known psychiatric disorders, including schizophrenia and depression. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit (recombinant)
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	4G3, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt 12.06 kDa, observed mol wt ~12 kDa
species reactivity	human, rat, mouse
packaging	antibody small pack of 25 µL
greener alternative product characteristics	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry,.
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
greener alternative category	Aligned,
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable