Anti-PDK4 Antibody, clone 2H5 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1921-4X25UL

ZRB1921-25UL

ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.Clone 2H5 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Pyruvate dehydrogenase kinase isoform 4 (PDK4). It targets an epitope within 18 amino acids from the N-terminal half.
KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal half of human Pyruvate dehydrogenase kinase isoform 4 (PDK4).
Quality Control Testing
Evaluated by Western Blotting in L6 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PDK4 in L6 cell lysate.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PDK4 in human skeletal muscle tissue lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected PDK4 in human heart tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound PDK4 peptide with a KD of 1.0 x 10-12 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PDK4 in HepG2 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-PDK4, clone 2H5 ZooMAb®, Cat. No. ZRB1921, is a recombinant Rabbit monoclonal antibody that specifically targets PDK4 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Target description
Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial (UniProt: Q16654; also known as EC:2.7.11.2, Pyruvate dehydrogenase kinase isoform 4, PDK4) is encoded by the PDK4 (also known as PDHK4) gene (Gene ID: 5166) in human. PDK4 is a homodimeric mitochondrial matrix enzyme that is ubiquitous in its distribution, but higher levels of expression are found in heart and skeletal muscle. It plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid (TCA) cycle and down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase is shown to reduce glucose utilization and increase fat metabolism in response to prolonged fasting and starvation. PDK4 plays a significant role in maintaining normal blood glucose levels under conditions of starvation. By regulating pyruvate dehydrogenase activity, it plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, it mediates cellular responses to glucose levels and to a high-fat diet. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	rabbit
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	2H5, recombinant monoclonal
description	recombinant, expressed in HEK 293 cells
product line	ZooMAb®
form	lyophilized
mol wt	calculated mol wt 46.47 kDa","observed mol wt ~52 kDa
species reactivity	human, rat
species reactivity (predicted by homology)	monkey, bovine
packaging	antibody small pack of 25 µL
enhanced validation	recombinant expression
application(s)	affinity binding assay: suitable","immunocytochemistry: suitable","immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable","western blot: suitable
isotype	IgG
epitope sequence	N-terminal half
conjugate	unconjugated
Protein ID accession no.	NP_002603.1,
UniProt accession no.	Q16654,
shipped in	ambient
storage temp.	2-8°C

WGK Germany

WGK 1