Anti-PGP9.5/UCHL1 Antibody, clone 3C13 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1664-4X25UL

ZRB1664-25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.,
Specificity
Clone 3C13 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects PGP 9.5 (PGP 9.5). It targets an epitope within 18 amino acids from the N-terminal region.
KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal region of human PGP 9.5 (UCHL1).
Quality Control Testing
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PGP9.5/UCHL1 in SH-SY5Y cell lysate.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected PGP9.5/UCHL1 in Human brain, mouse brain, rat brain tissue lysates and PC12 cell lysate.Affinity Binding Assay: A representative lot of this antibody bound PGP9.5 peptides with a KD of 7.5 x 10-8 in an affinity binding assay.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected PGP9.5/UCHL1 in human pancreas tissue sections.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PGP9.5/UCHL1 in SH-SY5Y cells.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-PGP9.5/UCHL1, clone 3C13 ZooMAb, Cat. No. ZRB1664, is a recombinant Rabbit monoclonal antibody that targets PGP9.5/UCHL1 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Target description
Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UniProt: P09936; also known as EC: 3.4.19.12, UCH-L1, Neuron cytoplasmic protein 9.5, PGP 9.5, PGP9.5, Ubiquitin thioesterase L1) is encoded by the UCHL1 gene (Gene ID: 7345) in human. UCH-L1 is a member of the peptidase C12 family that can exist as a monomer or homodimer. It is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors and is detected in neuronal cell bodies and processes throughout the neocortex. It displays a weak expression in ovary. About 30% of total UCH-L1 is reported to be associated with membranes in brain. Its levels are shown to be down regulated in brains from Parkinson disease and Alzheimer disease patients. UCH-L1 is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin and is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. It also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimeric form may have ATP-independent ubiquitin ligase activity. Mutations in UCHL1 gene are known to cause Parkinson disease 5, a complex neurodegenerative disorder with manifestations ranging from typical Parkinson disease to dementia with Lewy bodies. Some mutations in this gene are also linked to spastic paraplegia 79, where patients display a form of spastic paraplegia, which is characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200,
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
clone	recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt 24.82 kDa, observed mol wt ~ 25 kDa
species reactivity	rat, human, mouse
packaging	antibody small pack of 25 µL
greener alternative product characteristics	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry,.
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	affinity binding assay: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
greener alternative category	Aligned,
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable