Anti-PINK1 antibody produced in rabbit

MDL number:	MFCD08064431
NACRES:	NA.41

Кат. номер

P0076-200UL

P0076-25UL

PTEN induced putative kinase 1 (PINK1) is a serine/threonine kinase expressed in mitochondria. It contains an N-terminal mitochondrial targeting motif and a highly conserved kinase domain homologous to serine/threonine kinases of the Ca2+/calmodulin family.
Anti-PINK1 antibody produced in rabbit has been used in immunoblotting.
Biochem/physiol Actions
PINK1 (PTEN induced putative kinase 1, also known as PARK6 and BRPK), has been identified as linked to the autosomal recessive form of familial Parkinson disease (PD). PINK1 localized to the mitochondria protects cells from stress-induced mitochondrial dysfunction. Overexpression of wild-type PINK1 has been found to protect neurons from stress-induced mitochondrial dysfunction and apoptosis. Genetic studies in drosophila indicate that PINK1 acts upstream of Parkin in a common pathway that influences mitochondrial morphology. PINK1 activity has been shown to protect primary neurons in mouse from the dopaminergic neurotoxin MPTP (1-methyl-4-phenylpyridine (MPP+)/1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) both in vitro and in vivo. This protective activity requires PINK1 kinase activity, as a PINK1 G309D mutant linked to familial PD or a kinase dead mutant K219M are not protective. PINK1 has been shown to be cleaved and localized to the mitochondria, in response to enhanced proteasomal stress in vitro and correlates with increased expression of the processed PINK1 protein in PD brain.
Target description
PINK1 is a Ser/Thr kinase that has beenlocalized to the mitochondria, and thought to protectcells from stress-induced mitochondrial dysfunction.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	antigen ~60 kDa
species reactivity	rat (predicted), human, mouse (predicted)
enhanced validation	recombinant expression
concentration	~1.5 mg/mL
application(s)	western blot: 1-2 µg/mL using HEK-293T cell lysate expressign human PINK1
conjugate	unconjugated
UniProt accession no.	Q9BXM7,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... PINK1(65018) mouse ... Pink1(68943) rat ... Pink1(298575)

Personal Protective Equipment	Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US),
RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable