Anti-PINK1 (N-terminal region) antibody produced in rabbit

NACRES:

NA.41

Кат. номер

P0051-25UL

P0051-200UL

PTEN induced putative kinase 1 (PINK1) is a serine/threonine mitochondrial kinase. The 581 amino acid protein has an amino terminal mitochondrial target sequence, a putative transmembrane domain, a kinase domain and an autophosphorylation-regulating carboxy terminal domain. The gene encoding it is localized on human chromosome 1p36.12.
Anti-PINK1 (N-terminal region) antibody produced in rabbit has been used in western blotting.
Biochem/physiol Actions
PTEN induced putative kinase 1 (PINK1) has a role in removing damaged mitochondria from cells. Mitochondrial damage triggers the accumulation of the protein in the outer mitochondrial membrane, wherein it undergoes autophosphorylation and dimerizes into a supermolecular protein complex. PINK1 then phosphorylates ubiquitin and tags damaged mitochondria for mitophagy. It negatively modulates glioblastoma growth. Mutations in the PINK1 gene have been associated with recessive, early-onset Parkinsons disease. The protein is expressed in cancerous cells and has a role in cell survival.
PINK1 (PTEN induced putative kinase 1) has been found to protect neurons from stress-induced mitochondrial dysfunction and apoptosis. Genetic studies in Drosophila indicate that PINK1 acts upstream of Parkin in a common pathway that influences mitochondrial morphology. PINK1 elicits protection in mouse primary neurons from the dopaminergic neurotoxin 1-methyl-4-phenylpyridine (MPP+)/1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) both in vitro and in vivo. In response to enhanced proteasomal stress in vitro, PINK1 has been shown to be cleaved and localized to the mitochondria, and this correlates with increased expression of the processed PINK1 protein in Parkinsons disease(PD) brain.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	antigen ~60 kDa
species reactivity	human
packaging	antibody small pack of 25 µL
enhanced validation	recombinant expression
concentration	~1.5 mg/mL
application(s)	western blot: 1-2 µg/mL using HEK-293T cell lysate expressing human PINK1
conjugate	unconjugated
UniProt accession no.	Q9BXM7,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... PINK1(65018)

Personal Protective Equipment	Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US),
RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable