Anti-POR antibody produced in rabbit

Human Protein Atlas Number:

HPA010136    Human Protein Atlas characterization data

Кат. номер

HPA010136-100UL

HPA010136-25UL

Cytochrome p450 oxidoreductase (POR) is a 78 kDa membrane-bound diflavin oxidoreductase, which is located on the cytoplasmic side of the endoplasmic reticulum and the outer membrane of the nuclear envelope. The gene is located on human chromosome 7q11.23. POR consists of flavin mononucleotide (FMN) binding domain and nicotinamide adenine dinucleotide phosphate (NADPH)/flavin adenine dinucleotide (FAD) binding domain.
POR (P450 oxidoreductase) is an 80kDa membrane-bound enzyme, localized to endoplasmic reticulum (ER). It is a key enzyme in the P450-mon-oxygenase drug-metabolizing system. It belongs to the diflavin reductases.
NADPH--cytochrome P450 reductase recombinant protein epitope signature tag (PrEST)
Anti-POR antibody produced in rabbit has been used in elucidating the mechanisms responsible for the altered hepatic drug-metabolizing activity in acute lung injury (ALI) rats.
All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org),and as a result, are supported by the most extensive characterization in the industry.
The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these protocols, and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige,.
Biochem/physiol Actions
Cytochrome p450 oxidoreductase (POR) binds to two cofactors, FAD and FMN, which catalyses the transfer of reducing equivalents from NADPH to various physiological acceptors, such as cytochrome b5, squalene monooxygenase and heme oxygenase. It facilitates electron transfer from -NADPH to cytochrome P450 (CYP) in drug metabolism. Mutations in this gene is associated with congenital adrenal hyperplasia, Antley-Bixler syndrome (ABS), P450C17 and P450C21 deficiency, amenorrhea and polycystic ovaries.
POR (P450 oxidoreductase) functions as an electron donor for all microsomal CYP (cytochrome P450) enzymes. It plays an essential role in RA (retinoic acid) metabolism, as POR insufficient mice have markedly increased levels of RA. In human acute myeloid leukemia cells, the expression of POR is controlled by all-trans retinoic acid (ATRA) and by 1,25-dihydroxyvitamin D3. Studies in human cancer cell lines show that the activity of hypoxia-activated prodrugs (HAP) is dependent on POR enzyme.
Features and Benefits
Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.
Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Corresponding Antigen APREST71930,.
Physical form
Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide
Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	100
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
product line	Prestige Antibodies® Powered by Atlas Antibodies
form	buffered aqueous glycerol solution
species reactivity	rat, human, mouse
packaging	antibody small pack of 25 µL
enhanced validation	orthogonal RNAseq
application(s)	immunoblotting: 0.04-0.4 µg/mL","immunofluorescence: 0.25-2 µg/mL","immunohistochemistry: 1:200-1:500
immunogen sequence	KEEVPEFTKIQTLTSSVRESSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMSADPEEYDLADLSSLPEIDNALVVFCMATYGEGDPTDNAQDFYDWLQETDVDLSGVKFAVFGLGNKTYEHFNA
conjugate	unconjugated
UniProt accession no.	P16435,
shipped in	wet ice
storage temp.	20°C
Gene Information	human ... POR(5447)

Personal Protective Equipment	Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US),
RIDADR	NONH for all modes of transport
WGK Germany	WGK 1