Anti-PTRH1 Antibody, clone 3O6 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1162-25UL

ZRB1162-4X25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 3O6 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Probable peptidyl-tRNA hydrolase (PTRH1).
His-tagged full-length recombinant human Probable peptidyl-tRNA hydrolase (PTRH1).
Quality Control Testing
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PTRH1 in Jurkat cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PTRH1 in MCF-7, and Raw264.7 cell lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected PTRH1 in human adrenal gland tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound PTRH1 proteins with a KD of 1.1 x 10-7 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PTRH1 in MCF7 and Jurkat cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-PTRH1, clone 3O6 ZooMAb®, Cat. No. ZRB1162, is a recombinant Rabbit monoclonal antibody that specifically targets PTRH1 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Target description
Probable peptidyl-tRNA hydrolase (UniProt: Q86Y79; also known as EC:3.1.1.29, Pth) is encoded by the PTRH1 (also known as C9orf115) gene (Gene ID: 138428) in human. Peptidyl-tRNA hydrolase (PTRH) is a carboxylic ester hydrolase that releases tRNA from the premature translation termination product peptidyl-tRNA by cleaving the ester bond between the C-terminal end of the peptide and the 2 - or 3 -hydroxyl of the ribose at the end of the tRNA. During the protein translation process, a significant proportion of the ribosomes that initiate mRNA readout do not reach the stop codon and peptidyl-tRNA molecules may dissociate from the mRNA template causing a premature end of the process. Accumulation of peptidyl-tRNAs reduces the efficiency of translation by sequestering tRNAs and impairing the initiation. Action of PTRH relieves this inefficiency. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: de Pereda, JM et al., (2004). J. Biol. Chem. 279(9); 8111-8115; Das, G., and Varshney, U. (2006). Microbiology. 152(8); 2191 2195).
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200,
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	3O6, monoclonal, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt ~ 23 kDa
species reactivity	mouse, human
packaging	antibody small pack of 25 µL
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	affinity binding assay: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable