Anti-RAD21 Antibody, clone 2H12 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1458-4X25UL

ZRB1458-25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.,
Specificity
Clone 2H12 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects RAD21. It targets an epitope within 102 amino acids from the C-terminal half.
GST/His-tagged recombinant fragment corresponding to 102 amino acids from the C-terminal half of human Double-strand-break repair protein Rad21 homolog (RAD21).
Anti-RAD21, clone 2H12 ZooMAb, Cat. No. ZRB1458, is a recombinant Rabbit monoclonal antibody that specifically targets RAD21 and is tested for used in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RAD21 in NIH3T3 and L6 cell lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected RAD21 in human colon tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected RAD21 in A431, HeLa, HUVEC, Jurkat, K562, NIH3T3, and U2OS cells.
Affinity Binding Assay: A representative lot of this antibody bound recombinant RAD21 protein fragment with a KD of 9.0 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Double-strand-break repair protein rad21 homolog (UniProt: O60216; also known as hHR21, Nuclear matrix protein 1, NXP-1, SCC1 homolog) is encoded by the RAD21 (also known as HR21, KIAA0078, NXP1, SCC1) gene (Gene ID: 5885) in human. RAD21 is a double-strand-break repair protein rad21 homolog. It is a component of the cohesion complex and is integral to chromosome segregation, error-free DNA repair, and prevention of inappropriate recombination between repetitive regions. It is cleaved by separase/ESPL1 at the onset of anaphase and this cleavage is required for sister chromatid separation and cytokinesis. The cohesin complex also plays a role in spindle pole assembly during mitosis. RAD21 expression in colorectal cancer is associated with aggressive disease especially in KRAS mutant tumors and resistance to chemoradiotherapy. Mutations in RAD21 gene have been linked to Cornelia de Lange syndrome that is characterized by facial dysmorphisms, abnormal hands and feet, growth delay and cognitive retardation. Some mutations can also cause Mungan syndrome that leads to neuromyopathy, chronic intestinal pseudo-obstruction and a variety of cardiac valve defects. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Deb, S., et al. (2014). Br. J. Cancer 110(6); 1606-1613).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
0.03 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit (recombinant)
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	2H12, monoclonal, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt 71.69 kDa, observed mol wt 105 kDa
species reactivity	mouse, human, rat
packaging	antibody small pack of 25 µL
greener alternative product characteristics	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry,.
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	affinity binding assay: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
greener alternative category	Aligned,
shipped in	ambient
storage temp.	2-8°C

WGK Germany

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