Anti-RAR-alpha Antibody, clone 3L14, ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1369-25UL

ZRB1369-4X25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.,
Specificity
Clone 3L14 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Retinoic Acid Receptor alpha (RAR-alpha). It targets an epitope within 17 amino acids from the N-terminal half.
KLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal half of human Retinoic Acid Receptor alpha.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected RAR-alpha in A431 cell lysate.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected RAR-alpha in HeLa, A431, HUVEC, and NTERA2 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100-1,000 dilution from a representative lot detected RAR-alpha in human prostate cancer and human testis tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound Retinoic Acid Receptor alpha peptide with a KD of 9.8 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Anti-RAR-alpha, clone 3L14 ZooMAb, Cat. No. ZRB1369, a Rabbit monoclonal antibody that targets RAR-alpha and is tested in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Target description
Retinoic acid receptor alpha (UniProt: P10276; also known as RAR-alpha, Nuclear receptor subfamily 1 group B member 1) is encoded by the RARA (also known as NR1B1) gene (Gene ID: 5914) in human. RAR-alpha is a nuclear receptor of the steroid/thyroid superfamily of nuclear receptor proteins that regulate gene expression by binding to RNA and other elements of DNA response. RAR-alpha forms heterodimer with RXR (via C-terminus) and this association is enhanced by pulsatile shear stress. The RAR-alpha/RXR heterodimer bind to the retinoic acid response elements composed of tandem 5′-AGGTCA-3′ sites known as DR1-DR5. Its nuclear localization depends on ligand binding, phosphorylation, and sumoylation. Phosphorylation on serine 77 is shown to be crucial for its transcriptional activity. Phosphorylation by Akt1 is required for the repressor activity but it has no effect on DNA binding, protein stability, or subcellular localization. In the absence of ligand, the heterodimer associates with a multiprotein complex containing transcription corepressors that induces histone deacetylation, chromatin condensation and transcriptional suppression. Upon ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RAR-alpha has two NR C-4 type of zinc-finger domains (aa 88-108 and 124-148). This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit (recombinant)
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	3L14, monoclonal, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt 50.77 kDa, observed mol wt ~58 kDa
species reactivity	human, canine
packaging	pkg of 25 µL
greener alternative product characteristics	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry,.
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	affinity binding assay: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
greener alternative category	Aligned,
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable