Anti-Recoverin (C-terminal) antibody produced in rabbit SAB4200309-200UL

NACRES:

NA.44

The recoverin (RCVRN) gene is mapped to human chromosome 17p13.1. Recoverin is a neuronal calcium (Ca2+) sensor (NCS) protein and contains four EF-hand Ca2+-binding sites. Recoverin is mainly localized in the retina, in cone and rod photoreceptors, cone bipolar cells, and in a subpopulation of cells in the ganglion cell layer.
Specificity
Anti-Recoverin (C-terminal) specifically recognizes human and rat recoverin.
synthetic peptide corresponding to a sequence at the C-terminus of rat recoverin, conjugated to KLH. The corresponding sequence is identical in mouse recoverin and highly conserved (86% sequence identity) in human recoverin.
Anti-Recoverin (C-terminal) antibody produced in rabbit is suitable for immunoblotting.
Biochem/physiol Actions
Recoverin plays an important role in phototransduction. The neuronal calcium (Ca2+) sensor (NCS) proteins are involved in a variety of Ca2+-dependent signal transduction processes in neurons. Recoverin is expressed in early stages of development, thus providing an effective marker to assess the development of photoreceptors in the retina. Recoverin serves as a Ca2+ -dependent regulator of rhodopsin kinase (GRK1)/rhodopsin kinase that catalyzes the phosphorylation and desensitization of visual receptor rhodopsin. Recoverin has been suggested to interact with additional NCS proteins, including caldendrin, to modulate its activity. Recoverin has also been identified as an autoantigen in the degenerative disease of the retina cancer-associated neuropathy (CAR). The aberrant expression of recoverin in malignant tumors has been shown to cause an autoimmune response in some cancer patients that leads to the development of paraneoplastic CAR syndrome.
Physical form
Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	antigen ~23 kDa
species reactivity	rat, human
enhanced validation	recombinant expression
concentration	~1.5 mg/mL
application(s)	western blot: 1.5-3.0 µg/mL using rat brain extracts (S1 fraction) and SH-SY5Y cell extracts
conjugate	unconjugated
UniProt accession no.	P35243,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... RCVRN(5957) mouse ... Rcvrn(19674) rat ... Rcvrn(140936)

RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable