Anti-S100-B Antibody, clone 8F20 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1628-4X25UL

ZRB1628-25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.,
Specificity
Clone 8F20 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects S100-B.
His-tagged full-length recombinant human S100-B protein.
Quality Control Testing
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected S100-B in Rat Brain tissue lysate.Tested applicationsWestern Blotting Analysis: A 1:10,000 dilution from a representative lot detected S100-B in mouse spinal cord, human brain, and A375 lysates.Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected S100-B in human cerebellum tissue sections.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected S100-B in A375 cells.Flow Cytometry Analysis: 1 µg from a representative lot detected S100-B in one million C6 cells.Affinity Binding Assay: A representative lot of this antibody bound S-100 B protein with a KD of 3.2 x 10-7 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-S100-B, clone 8F20 ZooMAb, Cat. No. ZRB1628, is a recombinant Rabbit monoclonal antibody that specifically targets S100-B and is tested in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Target description
Protein S100-B (UniProt: P04271; also known as S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B) is encoded by the S100B gene (Gene ID: 6285) in human. S100-B is a member of the EF-hand calcium-binding protein superfamily that is widely distributed and conserved in the central nervous system of vertebrates. It exists in both heterodimeric and homodimeric forms. Existence of dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain has been reported and dimerization seems to be essential for its biological activities. S100-B has been suggested to play a part in a variety of cellular processes, primarily via binding to key synaptic proteins and inhibiting their phosphorylation. The extracellular form of S100-B, secreted by astrocytes, is physiologically involved in the development and maintenance of CNS homeostasis. S100-B effects are closely dependent on its cellular levels. At nanomolar concentrations it is shown to enhance survival of neurons during development and stimulate neurite outgrowth in cerebral cortex neurons. It also reduces the loss of mitochondrial function and cell death. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Donato, R. (2001). Int. J. Biochem. Cell Biol 33(7); 637-668; Rezaei, O., et al. (2017). Iran J. Neurol. 16(2); 83-89).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200,
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
clone	recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt 10.71 kDa, observed mol wt ~ 10 kDa
species reactivity	rat, human, mouse
packaging	antibody small pack of 25 µL
greener alternative product characteristics	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry,.
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	affinity binding assay: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
greener alternative category	Aligned,
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable