Anti-SCARB1 Antibody, clone 3B15 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1181-25UL

ZRB1181-4X25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.,
Specificity
Clone 3B15 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Scavenger receptor class B member 1 (SCARB1). It targets an epitope within 25 amino acids from the N-terminal region.
KLH-conjugated linear peptide corresponding to 25 amino acids from the N-terminal, extracellular domain of human Scavenger receptor class B member 1 (SCARB1).
Quality Control Testing
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SCARB1 in Human adrenal gland tissue lysate.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected SCARB1 in HeLa Membrane extract.Affinity Binding Assay: A representative lot of this antibody bound SCARB1 peptide with a KD of 5.8 x 10-9 in an affinity binding assay.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SCARB1 in human adrenal gland tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-SCARB1, clone 3B15 ZooMAb, Cat. No. ZRB1181, is a recombinant Rabbit monoclonal antibody that specifically targets SCARB1 and has been tested for use in Affinity Binding Assay, Immunohistochemistry (Paraffin), and Western Blotting.
Target description
Scavenger receptor class B member 1 (UniProt: Q8WTV0; also known as SCARB1, SRB1, CD36 and LIMPII analogous 1, CLA-1, CD36 antigen-like 1, Collagen type I receptor, thrombospondin receptor-like 1, SR-BI, CD36) is encoded by the SCARB1 (also known as CD36L1, CLA1) gene (Gene ID: 949) in human. SCARB1 is a widely expressed, multi-pass membrane glycoprotein with two cytoplasmic domains (aa 1-11 and 465-552), one extracellular domain (aa 33-443) and two transmembrane domains (aa 12-32 and 444-464). It serves as a receptor for several different ligands such as phospholipids, cholesterol ester, lipoproteins, and phosphatidylserine. It also serves as a receptor for HDL and mediates selective uptake of cholesteryl ether and HDL-dependent cholesterol efflux. It is reported to facilitate the flux of free and esterified cholesterol between the cell surface and ApoB-containing lipoproteins and modified lipoproteins, although less efficiently than HDL. SCRAB1 is also involved in phagocytosis of apoptotic cells, via its phosphatidylserine binding activity. Elevated expression of SCARB1 has been reported in a wide variety of malignant cell lines such as breast, prostate, ovarian, pancreatic, nasopharyngeal, and colorectal cancers. In hepatocytes, it is reported to act as a receptor for hepatitis C virus and facilitates virus entry into cells. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Mooberry, LK., et al. (2016). Front. Pharmacol. 7; 466).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200,
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
clone	recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt 60.88 kDa, observed mol wt ~ 65 kDa
species reactivity	human
packaging	antibody small pack of 25 µL
greener alternative product characteristics	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry,.
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	affinity binding assay: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
greener alternative category	Aligned,
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 2
Flash Point F	Not applicable
Flash Point C	Not applicable