Anti-SLC1A1 (C-terminal) antibody produced in rabbit SAB4200386-200UL

Solute carrier family 1 member 1 (SLC1A1) (also known as EAAC1, EAAT3) is localized at the postsynaptic site of neurons, whereas GLAST/EAAT1 and GLT1/EAAT2 are expressed at excitatory synapses by surrounding astrocytes. SLC1A1/EAAC1 is a glutamate transporter, expressed at moderate levels in the adult brain, mainly in the hippocampus, basal ganglia and olfactory bulb. The EAAT3 gene is mapped to human chromosome 9p24.2.
Specificity
Anti-SLC1A1(C-terminal) specifically recognizes human, rat and mouse SLC1A1.
synthetic peptide corresponding to a sequence at the C-terminal of human SLC1A1, conjugated to KLH. The corresponding sequence is identical in mouse and rat SLC1A1.
Anti-SLC1A1 (C-terminal) antibody produced in rabbit has been used in:

• immunocytochemistry
• immunoprecipitation
• immunoblotting
• immunofluorescence

Biochem/physiol Actions
Solute carrier family 1 member 1 (SLC1A1) neural activity is highly regulated by intracellular signaling pathways including protein kinase C (PKC ), phosphatidylinositol-3-kinase (PI3K), and glial factors, resulting in rapid changes in the trafficking of the transporter and its membrane location. It is considered to make a minor contribution to glutamate removal from the synapse and plays a key role in glutamate-mediated neuroplasticity. Hemi-deletion of the SLC1A1 gene may contribute to the pathophysiology of schizophrenia. The levels of EAAC1 are elevated in the plasma membrane when subjected to in vivo, long term potentiation or contextual fear conditioning. SLC1A1 displays increased expression in clear cell renal cell carcinoma (ccRCC).
Physical form
Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	antigen ~70 kDa
species reactivity	rat, human, mouse
enhanced validation	recombinant expression
concentration	~1.0 mg/mL
application(s)	indirect immunofluorescence: 2-4 µg/mL using Neuro-2a cells.","western blot: 1.5-3.0 µg/mL using postnatal (PN3) rat brain (S1 fraction) extracts, and 0.5-1.0 mg/mL using HEK-293T cell lysate over-expressing human SLC1A1.
conjugate	unconjugated
UniProt accession no.	P53814,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... SMTN(6525) mouse ... Smtn(29856) rat ... Smtn(289734)

RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable