Anti-SOX17 Antibody, clone 3F7, ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1345-4X25UL

ZRB1345-25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.,
Specificity
Clone 3F7 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects SOX-17. It targets an epitope within the C-terminal region.
His-tagged recombinant fragment corresponding to 149 amino acids from the C-terminal region of human SOX-17.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected SOX17 in human fallopian tube and human cervical tissue sections.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected SOX17 in human fallopian tube and human cervical tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound recombinant SOX17 with a KD of 5.0 x 10-8 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Anti-SOX17, clone 3F7 ZooMAb, Cat. No. ZRB1345, is a recombinant Rabbit monoclonal antibody that specifically targets SOX17 and has been tested for use in Affinity Binding Assay, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
Target description
Transcription factor SOX-17 (UniProt: Q9H6I2; also known as SOX-17) is encoded by the SOX17 gene (Gene ID: 64321) in human. SOX17 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. It binds to the sequences 5′-AACAAT-3 or 5′-AACAAAG-3′ and modulates transcriptional regulation via WNT3A. It is reported to act upstream of the Notch system and downstream of the canonical Wnt system and provides a link between the canonical Wnt and Notch signaling. It is involved in the regulation of the formation of cells that go on to develop into endodermal tissues, such as pancreas and liver. Although detected in multiple tissues, it is preferentially expressed in esophagus, stomach and small intestine. SOX17 required for normal looping of the embryonic heart tube and in embryonic and postnatal vascular development. Inactivation of SOX17 in endothelial cells in mouse embryo is reported to cause a lack of arterial differentiation and vascular remodeling resulting in death in utero. Embryonic stem cells that are deficient in SOX17 fail to differentiate into extraembryonic cell types and maintain expression of pluripotency-associated transcription factors. However, forced expression of SOX17 results in down-regulation of embryonic stem cell associated gene expression and activates genes that are involved in differentiation toward an extraembryonic endoderm cell fate. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Corada, M., et al. (2013). Nat. Commun. 4, 2609; Niakan, KK et al. (2010). Gene Dev. 24(3): 312-326).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit (recombinant)
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	3F7, monoclonal, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt 44.12 kDa, observed mol wt ~60 kDa
species reactivity	human, canine
packaging	pkg of 25 µL
greener alternative product characteristics	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry,.
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	affinity binding assay: suitable, immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
greener alternative category	Aligned,
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable