Anti-Substance P Antibody, clone 1K3 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1506-25UL

ZRB1506-4X25UL

ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 1K3 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Substance P.
KLH-conjugated linear peptide corresponding to full-length Substance P (11 amino acids).
Quality Control Testing
Evaluated by Western Blotting with recombinant Substance P (TAC1) with N-terminal His/ABP tags
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected recombinant Substance P (TAC1) with N-terminal His/ABP tags .
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Substance P in Jurkat cell lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Substance P in human cerebral cortex tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Substance P in SH-SY5Y cells.
Affinity Binding Assay: A representative lot of this antibody bound Substance P with a KD of 5.1 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Substance P, clone 1K3 ZooMAb®, Cat. No. ZRB1506, is a recombinant Rabbit monoclonal antibody that detects Substance P and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Target description
Substance P, a member of the tachykinin family, is an 11 amino acid peptide that is derived from the Protachykinin-1 (UniProt: P20366) that is encoded by TAC1 gene (Gene ID: 6863) in human. Protachykinin-1 is synthesized with a signal peptide (aa 1-19) and a propeptide (aa 20-56), which are subsequently cleaved off. The resulting peptide can be further cleaved into five different peptides- Substance P (aa 58-68), neuropeptide K (aa 72-107), neurokinin A (aa 98-107), neuropeptide gamma (aa 72-107) and C-terminal-flanking peptide (aa 111-126). Substance P is a potent hypotensive peptide that is most well-studied of the tachykinin family of peptides. Its actions are mediated via binding to NK1 receptors. Substance P is widely distributed in the central and peripheral nervous system of vertebrates. It is also found in association with nerves intrinsic to the gastrointestinal tract arising from the submucous and myenteric plexuses. In the CNS, it participates in various behavioral responses and in regulating neuronal survival and degeneration. It also regulates cardiovascular and respiratory function and is involved in activating the emetic reflex. In the spinal cord, substance P participates in neurotransmission of pain and noxious stimuli and modulates autonomic reflexes. It is also reported to elicit local vasodilatation and alter vascular permeability, which enhances the delivery and accumulation of leukocytes to tissues for the expression of local immune responses. It is also known to stimulate the chemotaxis of lymphocytes, monocytes, neutrophils, and fibroblasts. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: O Connor, TM., et al. (2004). Cell. Physiol. 201(2); 167-180).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	rabbit
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	1K3, recombinant monoclonal
description	recombinant, expressed in HEK 293 cells
product line	ZooMAb®
form	lyophilized
mol wt	calculated mol wt 1.35 kDa","observed mol wt ~30 kDa
purified by	using Protein A
species reactivity	human, rat, mouse
packaging	antibody small pack of 25 µL
enhanced validation	recombinant expression
application(s)	affinity binding assay: suitable","immunocytochemistry: suitable","immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable","western blot: suitable
isotype	IgG
epitope sequence	Unknown
conjugate	unconjugated
Protein ID accession no.	NP_003173,
UniProt accession no.	P20366,
shipped in	ambient
storage temp.	2-8°C