Anti-Synip (N-terminal) antibody produced in rabbit

NACRES:

NA.41

Кат. номер

S1700-200UL

S1700-25UL

Syntaxin-4 interacting protein (STXBP4) is also known as synip. It is expressed at high level in insulin-secreting pancreatic β cell line, βHC-9 cells. STXBP4 gene is located on human chromosome 17q22.
Specificity
Anti-Synip (N-terminal) specifically recognizes human Synip.
Anti-Synip (N-terminal) antibody produced in rabbit may be used in immunoblotting.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper),
Biochem/physiol Actions
Syntaxin-4 interacting protein (STXBP4) serve as functional links between the insulin signaling cascade and insulin-responsive glucose transporter (GLUT4) vesicles. It interacts with syntaxin 4. Synip inhibits the translocation of GLUT4 from intracellular vesicles to the plasma membrane by binding to syntaxin 4 and preventing the interaction between syntaxin 4 and vesicle-associated membrane protein (VAMP2). STXBP4 can control ΔNp63 ubiquitination. Hence it may act as a squamous cell carcinoma (SCC) biomarker.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	antigen ~65 kDa
species reactivity	human
packaging	antibody small pack of 25 µL
enhanced validation	recombinant expression
concentration	~1.5 mg/mL
application(s)	western blot: 0.2-0.4 µg/mL using HEK-293T cell lysate expressing human Synip and 0.3-0.6 mg/mL using a HepG2 cell lysate
conjugate	unconjugated
UniProt accession no.	Q6ZWJ1,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... STXBP4(252983)

Personal Protective Equipment	Eyeshields,, Gloves,, multi-purpose combination respirator cartridge (US),
RIDADR	NONH for all modes of transport
Flash Point F	Not applicable
Flash Point C	Not applicable