Anti-Synphilin-1 antibody produced in rabbit S5946-200UL

MDL number:	MFCD02264134
NACRES:	NA.41

Synphilin-1 (synuclein a-interacting protein 1, SNCAIP), is a cytoplasmic protein that interacts with α-synuclein in neurons. Synphilin-1 contains several protein-protein interaction domains, including six ankyrin-like repeats, coiled-coil domain, and an ATP/GTP binding domain. The synphilin gene produces at least nine transcript variants encoding seven distinct proteins. Synphilin transcripts are widely expressed in many tissues with highest levels found in the brain, heart and placenta. Synphilin-1 is highly enriched in presynaptic nerve terminals.
synthetic peptide corresponding to amino acids 829-847 of human synphilin, conjugated to KLH. This sequence is identical in dog synphilin-1 and highly conserved (84% identity) in mouse, rat, and bovine synphilin-1.
Anti-Synphilin-1 antibody produced in rabbit has been used in co-immunoprecipitation and western blotting.
Biochem/physiol Actions
Synphilin-1 associates with and is ubiquitinated by several proteins, including α -synuclein, parkin, dorfin and siah E3 ubiquitin protein ligase 1 (SIAH1), and is a major component of Lewy bodies in Parkinson′s disease (PD). It is associated with synaptic vesicles and is modulated by α-synuclein. The central domain of synphilin-1 has been suggested to be required for the formation of aggregates and cytotoxicity. Synphilin-1 association with α -synuclein promotes the formation of cytosolic inclusions. Mutation of synphilin-1 in PD patients at amino acid R621C, is implicated in these sequestration, ubiquitination and proteasomal inhibition leading to increased accumulation of toxic intermediates. Cells with this mutation are more prone to staurosporine-induced cell death highlighting its cytoprotective functionality. In human postmortem brain tissue, synphilin-1, like a-synuclein is present in neurophil.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200
antibody form	affinity isolated antibody
antibody product type	primary antibodies
clone	polyclonal
form	buffered aqueous solution
mol wt	antigen 130 kDa
species reactivity	human
enhanced validation	recombinant expression
concentration	~2 mg/mL
application(s)	western blot: 0.25-0.5 µg/mL using HEK-293 cells expressing human synphilin-1
conjugate	unconjugated
UniProt accession no.	Q9Y6H5,
shipped in	dry ice
storage temp.	20°C
Gene Information	human ... SNCAIP(9627) mouse ... Sncaip(67847) rat ... Sncaip(445354)

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable