Anti-Thy-1(CD90) Antibody, clone 1I7 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1285-25UL

ZRB1285-4X25UL

ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.Clone 1I7 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Thy-1 membrane glycoprotein (CD90). It targets an epitope within 19 amino acids from the internal region.
KLH-conjugated linear peptide corresponding to 19 amino acids from the internal region of human Thy-1 (CD90) membrane glycoprotein.
Anti-Thy-1(CD90), clone 1I7 ZooMAb®, Cat. No. ZRB1285, is a recombinant Rabbit monoclonal antibody that detects Thy-1 (CD90) and is tested in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Quality Control Testing
Evaluated by Western Blotting in SK-N-SH cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Thy-1(CD90) in SK-N-SH cell lysate.
Tested applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Thy-1(CD90) in K562 and TF-1 cell lysates.
Immunocytochemistry Analysis: A 1:1000 dilution from a representative lot detected Thy-1(CD90) in SK-N-SH cells.
Affinity Binding Assay: A representative lot of this antibody bound THY-1 (CD90) peptide with a KD of 2.1 x 10-7 in an affinity binding assay.
Flow Cytometry Analysis: 0.1 µg from a representative lot detected Thy-1(CD90) in one million TF1 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Thy-1(CD90) in human tonsil tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Thy-1 membrane glycoprotein (UniProt: P04216; also known as CDw90, Thy-1 antigen, CD90) is encoded by the THY1 gene (Gene ID: 7070) in human. Thy-1 is a highly conserved, GPI-anchored membrane protein with one Ig V-type superfamily domain (aa 20-126). It is synthesized with a signal peptide (aa 1-19) and a propeptide (aa 131-161) that are subsequently cleaved off to generate the mature form (aa 20-130). It lacks an intracellular domain but is located in the outer leaflet of lipid rafts at the cell plasma membrane allowing signaling functions by cis- and trans-interactions with G inhibitory proteins, the Src and c-fyn kinases, and tubulin. Thy-1 is a major cell surface protein found on both T-cells and in the brain. It plays a key role in cell-cell and cell-ligand interactions during synaptogenesis and other events in the brain. It also plays a role in the regulation of axon growth and nerve regeneration, T cell activation and apoptosis, leukocytes and melanoma cell adhesion and migration, and fibroblast proliferation and migration in wound healing. Thy-1 mRNA and protein expression has been reported in several cancer types including liver, myeloid, skin, and brain. It is considered as a marker for glioblastoma stem cells. Reduced expression of Thy-1 is shown to enhance the expression of osteogenic and adipogenic differentiation of mesenchymal stem cells. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Sauzay, C., et al. (2019). Front. Cell Dev. Biol. 7; 66; Moraes, DA., et al. (2016). Stem Cell Res. Ther. 7; Article 97).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality Level	200
biological source	rabbit
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	1I7, recombinant monoclonal
description	recombinant, expressed in HEK 293 cells
product line	ZooMAb®
form	lyophilized
mol wt	calculated mol wt 17.94 kDa","observed mol wt ~24 kDa
species reactivity	human
packaging	antibody small pack of 25 µL
enhanced validation	recombinant expression
application(s)	affinity binding assay: suitable","flow cytometry: suitable","immunocytochemistry: suitable","immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable","western blot: suitable
isotype	IgG
epitope sequence	Internal
conjugate	unconjugated
Protein ID accession no.	NP_006279.2,
UniProt accession no.	P04216,
shipped in	ambient
storage temp.	2-8°C