Anti-Treslin Antibody, clone 5L19 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1063-4X25UL

ZRB1063-25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.,
Specificity
Clone 5L19 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Treslin. It targets an epitope within 25 amino acids from the C-terminal region.
KLH-conjugated linear peptide corresponding to 25 amino acids from the C-terminal region of human Treslin.
Anti-Treslin, clone 5L19 ZooMAb, Cat. No. ZRB1063, is a recombinant Rabbit monoclonal antibody that specifically targets Treslin and is tested for use in Affinity Binding Assay, Immunocytochemistry, and Western Blotting.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Treslin in NIH 3T3 cells.
Affinity Binding Assay: A representative lot of this antibody bound Treslin peptides with a KD of 4.3 x 10-6 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Treslin (UniProt: Q7Z2Z1; also known as TopBP1-interacting checkpoint and replication regulator, TopBP1-interacting, replication-stimulating protein) is encoded by the TICRR (also known as C15orf42) gene (Gene ID: 90381) in human. Treslin is a well conserved nuclear protein that associates with replicating chromatin. It serves as a regulator of DNA replication by promoting integration of Cdc45 into the replicative helicase. It is a key positive-regulatory target of cell-cycle control mechanisms and activation of Treslin by cyclin-dependent kinase (CDK) is shown to be essential for the initiation of replication. It possesses an unusually large number of potential CDK phosphorylation sites. Checkpoint-regulatory kinase Chk1 is shown to associate with a C-terminal domain of Treslin (TRCT) and mutations in the TRCT domain are known to abolish binding of Chk1 to Treslin and thereby eliminate its phosphorylation by Chk1. Disruption of the Treslin-Chk1 interaction is shown to increase initiation of chromosomal DNA replication. Two isoforms of Treslin have been described that are produced by alternative splicing. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Kumagai, A., et al. (2010). Cell. 140(3); 349-359; Guo, C., et al. (2015). Mol. Cell. 57(3); 492-505).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200,
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	5L19, monoclonal, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt 210.86 kDa, observed mol wt ~260 kDa
species reactivity	mouse
species reactivity (predicted by homology)	human, monkey
packaging	antibody small pack of 25 µL
greener alternative product characteristics	Waste Prevention Designing Safer Chemicals Design for Energy Efficiency Learn more about the Principles of Green Chemistry,.
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	affinity binding assay: suitable, immunocytochemistry: suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
greener alternative category	Aligned,
shipped in	ambient
storage temp.	2-8°C

WGK Germany

WGK 1