Anti-Tubulin -2 Antibody, clone 1M19 ZooMAb® Rabbit Monoclonal

Кат. номер

ZRB1123-25UL

ZRB1123-4X25UL

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 1M19 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects human g-Tubulin. It targets an epitope within 16 amino acids from the C-terminal region.
KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal region of human g-Tubulin.
Anti-Tubulin g-2, clone 1M19 ZooMAb®, Cat. No. ZRB1123, is a recombinant Rabbit monoclonal antibody that specifically targets Tubulin g-2 and is tested for use in Affinity Binding Assay, Immunohistochemistry (Paraffin), and Western Blotting.
Quality Control Testing
Evaluated by Western Blotting in U2OS cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Tubulin g-2 in U2OS cell lysate.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Tubulin -2 in human colon tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound Tubulin peptide with a KD of 3.4 x 10-6 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Tubulin g-2 chain (UniProt: Q9NRH3; also known as Gamma-2-tubulin, Gamma tubulin) is encoded by the TUBG2 gene (Gene ID: 27175) in human. Tubulin is the major constituent of microtubules. In humans five isoforms of tubulin have been described and are known as -tubulin, -tubulin, -tubulin, -tubulin, and -tubulin. The g -chain is found at microtubule organizing centers such as the spindle poles or the centrosome. It is a component of the pericentriolar matrix that regulates / chain minus-end nucleation, centrosome duplication, and spindle formation. It is ubiquitously expressed and is abundant in both the cytosol and the nuclear compartments of cells in all mammalian tissues. By binding to the -tubulin subunit, it establishes the polarity of a microtubule leaving the -tubulin subunit exposed at the positive end. Although g-tubulin is reported to be only about 1% of the level of either or -tubulin, it shares approximately 28-32% identity with a-tubulin and 32-36% identity with -tubulin in various species. g -tubulin is reported to form two complexes known as the g-tubulin small complex and the larger g-tubulin ring complex and each complex contains a several g-tubulin complex proteins (GCPs). g -1 tubulin knockout mice survive only up to the morula/blastocyst stage; however, g-2 tubulin knockout mice remain viable and fertile. In human neuroblastoma cells, g-2 tubulin is shown to be a pro-survival factor and its levels are up-regulated during neural development and oxidative stress. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Alvarado-Kristensson, M. (2018). Signal Trans. Target. Ther. 3; Article 24).
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source	rabbit
Quality Level	200,
recombinant	expressed in HEK 293 cells
antibody form	purified antibody
antibody product type	primary antibodies
clone	1M19, monoclonal, recombinant monoclonal
product line	ZooMAb® learn more,
form	lyophilized
mol wt	calculated mol wt ~ 51.1 kDa, observed mol wt ~ 51 kDa
species reactivity	human
species reactivity (predicted by homology)	monkey
packaging	antibody small pack of 25 µL
enhanced validation	recombinant expression Learn more about Antibody Enhanced Validation,
application(s)	affinity binding assay: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
isotype	IgG
conjugate	unconjugated
shipped in	ambient
storage temp.	2-8°C

WGK Germany	WGK 1
Flash Point F	Not applicable
Flash Point C	Not applicable